Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-04T18:52:08.524Z Has data issue: false hasContentIssue false

Invasion of chicken caecal and intestinal lamina propria by crypt epithelial cells infected with coccidia

Published online by Cambridge University Press:  06 April 2009

M. A. Fernando
Affiliation:
Department of Pathology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, CanadaNIG 2 W1
A. M. Lawn
Affiliation:
Department of Houghton Poultry Research Station, Houghton, Huntingdon, Cambs. PE17 2DA
M. Elaine Rose
Affiliation:
Department of Houghton Poultry Research Station, Houghton, Huntingdon, Cambs. PE17 2DA
M. A. Al-Attar
Affiliation:
Department of Pathology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, CanadaNIG 2 W1

Summary

The development of second generation schizonts of Eimeria necatrix and E. tenella was studied with the electron microscope. Invasion of the crypt epithelial cells by merozoites of the first generation schizonts caused changes in the morphology of the infected cells and stimulated their migration into the lamina propria through breaks which appeared in the basement membrane of the crypts. Second generation schizonts developed in the lamina propria within these crypt cells whose epithelial origin was confirmed by their interconnection by desmosomes and tight junctions and by their possession of characteristic microvilli. A comparison is made between this invasion of the lamina propria by parasitized cells and invasion of connective tissue by malignant epithelial cells; the possible mechanisms involved are discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alroy, J. & Gould, V. E. (1980). Epithelial-stromal interface in normal and neoplastic human bladder epithelium. Ultrastructural Pathology 1, 201–10.CrossRefGoogle ScholarPubMed
Bergmann, V. (1970). Elektronenmikroskopische Untersuehungen zur Pathogenese der Blind-darmkokzidiose der Hühnerkuken. Arkiv für experimentelle Veterinärmedizin 24, 1170–84.Google Scholar
Beyer, T. V. & Shibalova, T. A. (1974). The increase of DNA quantity in the nuclei of chicken cecal cells harbouring second generation schizonts of Eimeria tenella. Parasitologia (Leningrad) 8, 449–55. (In Russian.)Google Scholar
Browning, R. F., Patton, W. H. & Lytle, C. F. (1976). Eimeria tenella: stimulation of DNA synthesis in infected cultured animal cells. Experimental Parasitology 39, 195203.CrossRefGoogle ScholarPubMed
Culling, C. G. A. (1974). Handbook of Histopathological and Histochemical Techniques. London: Butterworth and Company.Google Scholar
Fernando, M. A. & Pasternak, J. (1977 a). Isolation of chick intestinal cells infected with second generation schizonts of Eimeria necatrix. Parasitology 74, 1926.CrossRefGoogle ScholarPubMed
Fernando, M. A. & Pasternak, J. (1977 b). Isolation of host-cell nuclei from chick intestinal cells infected with second generation schizonts of Eimeria necatrix. Parasitology 74, 2732.CrossRefGoogle ScholarPubMed
Fernando, M. A., Pasternak, J., Barrell, R. & Stockdale, P. H. G. (1974). Induction of host nuclear DNA synthesis in coccidia infected chicken intestinal cells. International Journal for Parasitology 4, 267–76.CrossRefGoogle ScholarPubMed
Fernando, M. A. & Stockdale, P. H. G. (1974). Fine structural changes associated with schizogony in Eimeria necatrix. Zeitschrift für Parasitenkunde 43, 105–14.CrossRefGoogle ScholarPubMed
Hashimoto, K., Yamanishi, Y. & Dabbous, M. K. (1972). Electron microscopic observations of collagenolytic activity of basal cell epithelioma of the skin in vivo and in vitro. Cancer Research 32, 2561–7.Google ScholarPubMed
Hashimoto, K., Yamanishi, Y., Maeyens, E., Dabbous, M. K. & Kanxaki, T. (1973). Collagenolytic activities of squamous cell carcinoma of the skin. Cancer Research 33, 27902801.Google ScholarPubMed
Kefalides, N. A. (1975). Basement membranes: structural and biosynthetic considerations. Journal of Investigative Dermatology 65, 8592.CrossRefGoogle ScholarPubMed
Latter, V. S. (1977). Nucleic acid metabolism of Eimeria growing in cultivated cells. Fifth International Congress of Protozoology. New York, pp. 380.Google Scholar
Lee, D. L. & Long, P. L. (1972). An electron microscopal study of Eimeria tenella growth in the liver of the chick embryo. International Journal for Parasitology 2, 214–15.CrossRefGoogle ScholarPubMed
Long, P. L. (1970). In vitro culture of Eimeria tenella. Journal of Parasitology 56, 214–15.Google Scholar
Mareel, M. C. (1980). Recent aspects of tumour invasiveness. International Review of Experimental Pathology 22, 65129.Google Scholar
McNutt, N. A. (1976). Ultrastructural comparison of the interface between epithelium and stroma in basal cell carcinoma and control human skin. Laboratory Investigation 35, 132-–2.Google ScholarPubMed
Ozello, L. (1974). Electron microscopic study of functional and dysfunctional human mammary glands. Journal of Investigative Dermatology 63, 1926.CrossRefGoogle Scholar
Ozello, L. & Santipak, P. (1970). Epithelial-stromal junction of intraductal carcinoma of the breast. Cancer 26, 1186–98.3.0.CO;2-C>CrossRefGoogle Scholar
Rubio, C. A. & Biberfeld, P. (1975). The basement membrane of the uterine cervix in dysplasia and squamous carcinoma: an immunofluorescent study with antibodies to basement membrane antigen. Acta Pathologica et Microbiologica Scandinavica Sect. A 83, 744–8.CrossRefGoogle ScholarPubMed
Stockdale, P. H. G. & Fernando, M. A. (1975). The development of the lesions caused by second generation schizonts of Eimeria necatrix. Research in Veterinary Science 19, 204–8.CrossRefGoogle ScholarPubMed
Sugar, F. (1968). An electron microscopic study of early invasive growth in human skin tumours and laryngeal carcinoma. European Journal of Cancer 4, 33–8.CrossRefGoogle ScholarPubMed
Thompson, J. E., Fernando, M. A. & Pasternak, J. (1979). Induction of gel-phase lipid in plasma membrane of chick intestinal cells after coccidial infection. Biochimica et biophysica acta 555, 472–84.CrossRefGoogle ScholarPubMed
Tyzzer, E. E. (1929). Coccidiosis in gallinaceous birds. American Journal of Hygiene 10, 269383.Google Scholar
Tyzzer, E. E., Theiler, H. & Jones, E. E. (1932). Coccidiosis in gallinaceous birds. II. A comparative study of species of Eimerio of the chicken. American Journal of Hygiene 15, 319–93.Google Scholar
Urquhart, C. (1981 a). Thymidine uptake by chicken kidney cells parasitized by Eimeria tenella: evidence for a diffusable mediating substance. Parasitology 82, 189–94.CrossRefGoogle ScholarPubMed
Urquhart, C. (1981 b). Morphological and adhesive changes to cultured chick kidney cells following parasitization with Eimeria tenella (Protozoa:Coccidia). Parasitology 82, 175–87.CrossRefGoogle Scholar
Walker, F. (1972 a). Basement-membrane turnover in the rat. Journal of Pathology 107, 119–21.CrossRefGoogle ScholarPubMed
Walker, F. (1972 b). Basement-membrane turnover in man. Journal of Pathology 107, 123–5.CrossRefGoogle ScholarPubMed
Woods, D. A. & Smith, C. J. (1969). Ultrastructure of the dermal–epidermal junction in experimentally induced tumours and human oral lesions. Journal of Investigative Dermatology 52, 259–63.CrossRefGoogle ScholarPubMed