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Intestinal parasitic infections in an industrialized country; a new focus on children with better DNA-based diagnostics

Published online by Cambridge University Press:  23 August 2011

JACO J. VERWEIJ  and
LISETTE VAN LIESHOUT
JACO J. VERWEIJ*
Affiliation:
Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
LISETTE VAN LIESHOUT
Affiliation:
Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
*
*Corresponding author: Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Tel: +31 71 5265080. Fax: +31 71 5265069. E-mail: [email protected]
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Summary

In recent years, the isolation of parasitic DNA from faecal samples and PCR techniques, have been improved and simplified. Moreover, the introduction of real-time PCR has made it possible to multiplex different targets into one reaction. These new technical possibilities make it feasible to introduce PCR with its unsurpassed sensitivity and specificity in a routine laboratory setting for the diagnosis of intestinal parasites. Detection rates of the parasitic infections included in the PCR are increased significantly compared with microscopy. Molecular diagnostics, especially in children, reveal a possible cause of the gastrointestinal complaints in many more cases compared with conventional methods. Usually in GP patients no other pathogenic parasites are detected using microscopy and in the returning travellers additional parasites are found with microscopy in a minority of cases only. Multiplex real-time PCR offers a highly sensitive and specific diagnostic alternative for labour intensive microscopy in clinical laboratory practice. Additional diagnostic methods for the detection of parasitic infections that are not included as PCR target can be limited to a selected group of patients.

Keywords

G. lambliaCryptosporidiumD. fragilisE. histolyticachildrenreal-time PCR
Type
Research Article
Information
Parasitology , Volume 138 , Special Issue 12: Symposia of the British Society for Parasitology Volume 47 , October 2011 , pp. 1492 - 1498
Copyright
Copyright © Cambridge University Press 2011

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