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Interferon-γ induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases

Published online by Cambridge University Press:  01 March 2000

M. HAMADIEN
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
M. BAKHIET
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
R. A. HARRIS
Affiliation:
Neuroimmunology Unit, Centre for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden

Abstract

Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-γ. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-γ in a dose–response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-γ-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.

Type
Research Article
Copyright
2000 Cambridge University Press

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