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The interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticle

Published online by Cambridge University Press:  28 November 2001

S. MOHAN ,
S. FOULD  and
K. G. DAVIES
S. MOHAN
Affiliation:
IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK Indian Agricultural Research Institute, New Delhi, 110012, India
S. FOULD
Affiliation:
UMR 5557 Laboratoire d'Ecologie Microbienne 43 Quai du 11 Novembre 1918, 69622 Villeubanne Cedex France
K. G. DAVIES
Affiliation:
IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK
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Abstract

Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding domain (HBD) and the 45 kDa gelatin-binding domain (GBD) fragments of human fibronectin (Fn) revealed a series of polypeptides of approximately 40, 45 and 55 kDa present in crude cuticle extracts of Meloidogyne javanica 2nd-stage juveniles. The results suggest that the structure of the nematode fibronectin is different to the fibronectins so far characterized. Pre-treatment of endospores of Pasteuria with either the HBD or the GBD was found to inhibit binding to the nematode cuticle. The larger GBD fragment was the most effective at blocking adhesion. Pre-treatment of the GBD fragment with gelatin prevented the GBD fragment from inhibiting endospore attachment to the nematode cuticle.

Keywords

NematodaMeloidogyne javanicabacterial adhesionextracellular matrixbiological control
Type
Research Article
Information
Parasitology , Volume 123 , Issue 3 , March 2001 , pp. 271 - 276
Copyright
© 2002 Cambridge University Press

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References

AKIYAMA, S. K. & YAMADA, K. M. (1995). Fibronectin and fibronectin fragments. In Extracellular Matrix: a Practical Approach (ed. HARALSON, M. A. & HASSELL, J. R.), pp. 175–185. IRL Press at Oxford University Press, Oxford.
COURTNEY, H. S., HASTY, D. L. & OFEK, I. (1990). Hydrophobicity of group A streptococci and its relationship to adhesion of streptococci to host cells. In Microbial Cell Hydrophobicity (ed. DOYLE, R. I. & ROSENBERG, M.), pp. 361–386. American Society for Microbiology, Washington.
DAVIES, K. G., AFOLABI, P. & O'SHEA, P. S. (1996). Adhesion of Pasteuria penetrans to the cuticle of root-knot nematodes (Meloidogyne spp.) inhibited by fibronectin: a study of electrostatic and hydrophobic interactions. Parasitology 112, 553–559.Google Scholar
DAVIES, K. G. & BEADLE, J. (1995). Use of a cellulose acetate electrophoresis system for the simultaneous characterisation of individual root-knot (Meloidogyne spp.) females using three isozymes. Fundamental and Applied Nematology 18, 549–551.Google Scholar
DAVIES, K. G. & DANKS, C. (1993). Carbohydrate/protein interactions between the cuticle of infective juveniles of Meloidogyne incognita and spores of the obligate hyperparasite Pasteuria penetrans. Nematologica 39, 53–64.CrossRefGoogle Scholar
DAVIES, K. G., FARGETTE, M., BALLA, G., DAUDI, A., DUPONNOIS, R., GOWEN, S. R., MATEILLE, T., PHILLIPS, M. S., SAWADOGO, A., TRIVINO, C., VOUYOUKALOU, E. & TRUDGILL, D. L. (2000). Cuticle heterogeneity as exhibited by Pasteuria spore attachment is not linked to the phylogeny of parthenogenetic root-knot nematodes (Meloidogyne spp.) Parasitology 122, 111–120.Google Scholar
DAVIES, K. G., KERRY, B. R. & FLYNN, C. A. (1988). Observations on the pathogenicity of Pasteuria penetrans, a parasite of root-knot nematodes. Annals of Applied Biology 112, 1491–1501.CrossRefGoogle Scholar
DAVIES, K. G. & REDDEN, M. (1997). Diversity and partial characterisation of putative virulence determinants in Pasteuria penetrans, the hyperparasite of root-knot nematodes. Journal of Applied Microbiology 83, 227–235.CrossRefGoogle Scholar
DAVIES, K. G., REDDEN, M. & PEARSON, T. K. (1994). Endospore heterogeneity in Pasteuria penetrans related to attachment to plant-parasitic nematodes. Letters in Applied Microbiology 19, 370–373.CrossRefGoogle Scholar
DEITSCH, K. W., MOXON, E. R. & WELLEMS, T. E. (1997). Shared themes of antigenic variation and virulence in bacterial, protozoal, and fungal infections Microbiology and Molecular Biology Reviews 61, 281–282.Google Scholar
HASTY, D. L., BEACHEY, E. H., COURTNEY, H. S. & SIMPSON, W. A. (1989). Interactions between fibronectin and bacteria. In Fibronectin in Health and Disease (ed. CARSONS, S. E.), pp. 89–112. CRC Press. Boca Raton, Florida.
HATEFI, Y. & HANSTEIN, W. G. (1969). Solubilization of particulate proteins and non-electrolytes by chaotropic agents. Proceedings of the National Academy of Sciences, USA 62, 1129–1136.CrossRefGoogle Scholar
HEMMER, R. M., DONKIN, S. G., CHIN, K. J., GRENACHE, D. G., BHATT, H. & POLITZ, S. M. (1991). Altered expression of an L1-specific, O-linked cuticle surface glycoprotein in mutants of the nematode Caenorhabditis elegans. Journal of Cell Biology 115, 1237–1247.CrossRefGoogle Scholar
HEWLETT, T. E & DICKSON, D. W. (1993). A centrifuge method for attaching endospores of Pasteuria spp. to nematodes. Journal of Nematology 25(Suppl.), 785–788.Google Scholar
HOOPER, D. J. (1986). Extraction of nematodes from plant material. In Laboratory Methods for Working with Plant and Soil Nematodes (ed. SOUTHEY, J. F.), pp. 51–58. Ministry of Agriculture Fisheries and Food, London, H.M.S.O.
HYNES, R. O. (1990). Fibronectins. Springer-Verlag, New York.CrossRef
ISAACS, B. S., BREW, S. A. & INGHAM, K. C. (1989). Reversible unfolding of the gelatin-binding domain of fibronectin: structural stability in relation to function. Biochemistry 28, 842–850.CrossRefGoogle Scholar
PATTI, J. M., ALLEN, B. L., MCGAVIN, M. J. & HÖÖK, M. (1994). MSCRAMM-mediated adherence of microorganisms to host tissues. Annual Review of Microbiology 48, 585–617.CrossRefGoogle Scholar
PERSIDIS, A., LAY, J. G., MANOUSIS, T., BISHOP, A. H. & ELLAR, D. J. (1992). Characterisation of potential adhesins of the bacterium Pasteuria penetrans, and of putative receptors on the cuticle of Meloidogyne incognita, a nematode host. Journal of Cell Science 100, 613–622.Google Scholar
PETERSEN, T. E., THOGERSEN, H. C., SKORSTENGAARD, K., VIBEPEDERSEN, K., SAHL, P., SOTTRUPJENSEN, L. & MAGNUSSON, S. (1983). Partial primary structure of bovine plasma fibronectin – 3 types of internal homology. Proceedings of the National Academy of Sciences, USA 80, 137–141.CrossRefGoogle Scholar
POTTS, J. R. & CAMPBELL, I. D. (1994). Fibronectin structure and assembly. Current Opinion in Cell Biology 6, 648–655.CrossRefGoogle Scholar
POTTS, J. R. & CAMPBELL, I. D. (1996). Structure and function of fibronectin modules. Matrix Biology 15, 313–320.CrossRefGoogle Scholar
REDDIGARI, S. R., JANSMA, P. L., PREMACHANDRAN, D. & HUSSEY, R. S. (1986). Cuticular collagenous proteins of second-stage juveniles and adult females of Meloidogyne incognita: isolation and partial characterisation. Journal of Nematology 18, 294–302.Google Scholar
SAYRE, R. M. & STARR, M. P. (1988). Bacterial diseases and antagonisms of nematodes. In Diseases of Nematodes, Vol. 1, (ed. POINAR, G. O. & JANSSON, H. B. JR.), pp. 69–101. CRC Press, Boca Raton, Florida.
SKORSTENGAARD, K, HOLTET, T. L., ETZERODT, M. & THOGERSEN, H. C. (1994). Collagen-binding recombinant fibronectin fragments containing type-II domains. FEBS Letters 343, 47–50.CrossRefGoogle Scholar
STIRLING, G. R. (1991). Biological Control of Plant-Parasitic Nematodes: Progress, Problems and Prospects. CAB International, Wallingford.
YU, J. L., JOHANSSON, S. & LJUNGH, A. (1997). Fibronectin exposes different domains after adsorption to a heparinized and unheparinized poly(vinyl chloride) surface. Biomaterials 18, 421–427.CrossRefGoogle Scholar