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Influence of parasitization by Trichinella spiralis on the levels of heat shock proteins in rat liver and muscle

Published online by Cambridge University Press:  01 February 1999

J. MARTINEZ ,
J. PEREZ SERRANO ,
W. E. BERNADINA  and
F. RODRIGUEZ-CAABEIRO
J. MARTINEZ
Affiliation:
Faculty of Pharmacy, Department of Microbiology and Parasitology, University of Alcala, 28871 Alcala de Henares, Madrid, Spain
J. PEREZ SERRANO
Affiliation:
Faculty of Pharmacy, Department of Microbiology and Parasitology, University of Alcala, 28871 Alcala de Henares, Madrid, Spain
W. E. BERNADINA
Affiliation:
Faculty of Pharmacy, Department of Microbiology and Parasitology, University of Alcala, 28871 Alcala de Henares, Madrid, Spain Present address: Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, P.O. Box 80165, 3508 TD Utrecht, The Netherlands.
F. RODRIGUEZ-CAABEIRO
Affiliation:
Faculty of Pharmacy, Department of Microbiology and Parasitology, University of Alcala, 28871 Alcala de Henares, Madrid, Spain
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Abstract

To examine levels of heat shock proteins (HSPs) in host responses to helminth infection, rats were infected with Trichinella spiralis. The kinetics of HSP25, HSP60, HSP70 and HSP90 production in the liver and muscle of infected rats was compared with that of non-infected controls. HSPs were detected using electrophoretic analysis of fixed amounts of proteins (0·02 mg) and then blotting and incubation of membranes with polyclonal anti-HSP25 antibody, or monoclonal antibody against HSP60, or HSP70 or HSP90. Quantitation of blotted separated polypeptides reactive with the specific anti-HSP antibodies was achieved using an image analyser. Enhancement of HSP25 production was observed in the liver of infected rats, whereas muscle from the same rats exhibited enhanced production of HSP25 and HSP60 one day after infection only. These data indicate that HSPs levels can be used successfully to measure stress injury brought about by helminth infection in organs and tissues of the host.

Keywords

heat shock proteinnematodeTrichinella spiralis
Type
Research Article
Information
Parasitology , Volume 118 , Issue 2 , February 1999 , pp. 201 - 209
Copyright
1999 Cambridge University Press

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