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In vitro ovicidal and larvicidal activities of some saponins and flavonoids against parasitic nematodes of goats

Published online by Cambridge University Press:  21 May 2018

Acidália Carine Vieira Santos ,
Francianne Oliveira Santos ,
Hélimar Gonçalves Lima ,
Gisele Dias Da Silva ,
Rosangela Soares Uzêda ,
Êuder Reis Dias ,
Alexsandro Branco ,
Klauber Viana Cardoso ,
Jorge Mauricio David  and
Mariana Borges Botura
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Acidália Carine Vieira Santos
Affiliation:
Laboratório de Toxicologia, Hospital de Medicina Veterinária, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Francianne Oliveira Santos
Affiliation:
Laboratório de Toxicologia, Hospital de Medicina Veterinária, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Hélimar Gonçalves Lima
Affiliation:
Laboratório de Toxicologia, Hospital de Medicina Veterinária, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Gisele Dias Da Silva
Affiliation:
Laboratório de Toxicologia, Hospital de Medicina Veterinária, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Rosangela Soares Uzêda
Affiliation:
Departamento de Medicina Veterinária Preventiva e Produção Animal, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Êuder Reis Dias
Affiliation:
Laboratório de Fitoquímica, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, CEP: 44036-900, Feira de Santana, BA, Brazil
Alexsandro Branco
Affiliation:
Laboratório de Fitoquímica, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, CEP: 44036-900, Feira de Santana, BA, Brazil
Klauber Viana Cardoso
Affiliation:
Instituto de Química, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Jorge Mauricio David
Affiliation:
Instituto de Química, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
Mariana Borges Botura
Affiliation:
Laboratório de Toxicologia, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, CEP: 44036-900, Feira de Santana, BA, Brazil
Silvia Lima Costa
Affiliation:
Laboratório de Neuroquímica e Biologia Celular, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s/n, Vale do Canela, CEP: 40110-100, Salvador, BA, Brazil
Maria José Moreira Batatinha*
Affiliation:
Laboratório de Toxicologia, Hospital de Medicina Veterinária, Universidade Federal da Bahia, Av. Ademar de Barros, 500, Ondina, CEP: 40170-110, Salvador, BA, Brazil
*
Author for correspondence: Maria José Moreira Batatinha, E-mail: [email protected]
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Abstract

This study assessed the anthelmintic activity of plant-derived compounds against gastrointestinal nematodes of goats using the egg hatch and larval motility assays. The compounds tested were saponins (digitonin and aescin) and their respective sapogenins (aglycones), hecogenin acetate and flavonoids (catechin, hesperidin, isocordoin and a mixture of isocordoin and cordoin). Additionally, cytotoxicity of active substances was analysed on Vero cell through 3-4,5-dimethylthiazol-2-yl,2,5diphenyltetrazolium bromide (MTT) and propidium iodide (PI) tests. Significant reduction on the egg hatching (P < 0.05) was seen only in the treatments with aescin (99%/EC50 = 0.67 mg mL−1) and digitonin (45%). The compounds that reduced the larval motility (P < 0.05) were digitonin (EC50 = 0.03 mg mL−1 and EC90 = 0.49 mg mL−1) and the hecogenin acetate (75%). The other sapogenins showed low anthelmintic activity. All the flavonoids showed low ovicidal (4–12%) and larvicidal (10–19%) effects. The aescin and digitonin showed low toxicity in PI test (viable cells >90%). Nevertheless, higher cytotoxicity was observed in the MTT assay, with IC50 of 0.20 mg mL−1 (aescin) and 0.0074 mg mL−1 (digitonin). Aescin and digitonin have a pronounced in vitro anthelmintic effect and the glycone portion of these saponins plays an important role in this activity.

Keywords

Anthelminticflavonoidsapogeninsaponintoxicity
Type
Research Article
Information
Parasitology , Volume 145 , Issue 14 , December 2018 , pp. 1884 - 1889
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Copyright
Copyright © Cambridge University Press 2018 

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