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In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
B. C. Barr ,
K. W. Sverlow ,
M. Anderson ,
B. Daft ,
H. Kinde ,
J. P. Dubey ,
L. Munson  and
A. Ardans
P. A. Conrad
Affiliation:
Department of Veterinary Microbiology and Immunology, University of California, Davis, CA 95616, USA
B. C. Barr
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
K. W. Sverlow
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
M. Anderson
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
B. Daft
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
H. Kinde
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
J. P. Dubey
Affiliation:
Zoonotic Diseases Laboratory, Livestock and Poultry Sciences Institute, BARC-East, ARS, USD A, Beltsville, MD 20705, USA
L. Munson
Affiliation:
Department of Pathobiology, University of Tenessee, P.O. 1071, Knoxville, TN 37901, USA
A. Ardans
Affiliation:
California Veterinary Diagnostic Laboratory System (CVDLS), University of California, Davis, CA 95616, USA
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Summary

A Neospora sp. was isolated from the brains of two aborted bovine foetuses and grown continuously in vitro in bovine cell cultures. A comparison of the antigenic reactivity of in vitro cultivated tachyzoites with polyclonal antisera to Neospora caninum, Hammondia hammondi or Toxoplasma gondii revealed that the bovine protozoal isolates were similar to N. caninum and antigenically distinct from T. gondii. Tachyzoites of both bovine isolates had similar ultrastructural features, including an apical polar ring, conoid, electron-dense rhoptries and micronemes. The orientation of the micronemes, presence of micropores and a large number of electron-dense granules in the posterior portion of the bovine isolate tachyzoites differed from previous descriptions of N. caninum in vivo. Tachyzoites of the bovine isolates were ultrastructurally more similar to in vitro cultivated N. caninum tachyzoites than to tachyzoites of T. gondii or H. hammondi. The antigenic and ultrastructural similarities between N. caninum and the protozoal parasites isolated from aborted bovine foetuses in this study support the proposition that these parasites belong to the genus Neospora.

Keywords

NeosporaToxoplasmaHammondiaSarcocystisabortionultrastructure
Type
Research Article
Information
Parasitology , Volume 106 , Issue 3 , April 1993 , pp. 239 - 249
Copyright
Copyright © Cambridge University Press 1993

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