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The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients

Published online by Cambridge University Press:  06 April 2009

E. H. Carter ,
A. Suhrbier ,
P. J. A. Beckers  and
R. E. Sinden
E. H. Carter
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
A. Suhrbier
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
P. J. A. Beckers
Affiliation:
Department of Medical Parasitology, University of Nijmegen, Netherlands
R. E. Sinden*
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
*
*Reprint requests to Dr R. E. Sinden.
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Summary

Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0·45% become mature ookinetes. A single-step gradient centrifugation method on 12·5% Nycodenz® enriches both gametocytes and ookinetes.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 1 , August 1987 , pp. 25 - 30
Copyright
Copyright © Cambridge University Press 1987

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