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In vitro anti-Acanthamoeba activity of flavonoid glycosides isolated from Delphinium gracile, D. staphisagria, Consolida oliveriana and Aconitum napellus

Published online by Cambridge University Press:  24 June 2021

Rubén Martín-Escolano Open the ORCID record for Rubén Martín-Escolano [Opens in a new window] ,
Sonia Molero Romero ,
Jesus G. Díaz ,
Clotilde Marín Open the ORCID record for Clotilde Marín [Opens in a new window] ,
Manuel Sánchez-Moreno  and
Mª José Rosales
Rubén Martín-Escolano
Affiliation:
Laboratory of Molecular & Evolutionary Parasitology, RAPID group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
Sonia Molero Romero
Affiliation:
Departamento de Parasitología, Instituto de Investigacion Biosanitaria Ibs, University of Granada, Severo Ochoa s/n, E-18071, Granada, Spain
Jesus G. Díaz
Affiliation:
Departamento de Química Orgánica, Instituto de Bio-Orgánica ‘Antonio González’, Universidad de La Laguna, Ctra. a la Esperanza 2, 38206-La Laguna, Tenerife, Spain
Clotilde Marín
Affiliation:
Departamento de Parasitología, Instituto de Investigacion Biosanitaria Ibs, University of Granada, Severo Ochoa s/n, E-18071, Granada, Spain
Manuel Sánchez-Moreno
Affiliation:
Departamento de Parasitología, Instituto de Investigacion Biosanitaria Ibs, University of Granada, Severo Ochoa s/n, E-18071, Granada, Spain
Mª José Rosales*
Affiliation:
Departamento de Parasitología, Instituto de Investigacion Biosanitaria Ibs, University of Granada, Severo Ochoa s/n, E-18071, Granada, Spain
*
Author for correspondence: Mª José Rosales, E-mail: [email protected]
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Abstract

Acanthamoeba spp. are widely distributed in the environment and cause serious infections in humans. Treatment of Acanthamoeba infections is very challenging and not always effective which requires the development of more efficient drugs against Acanthamoeba spp. The purpose of the present study was to test medicinal plants that may be useful in the treatment of Acanthamoeba spp. Here we evaluated the trophozoital and cysticidal activity of 13 flavonoid glycosides isolated from Delphinium gracile, D. staphisagria, Consolida oliveriana and from Aconitum napellus subsp. Lusitanicum against the amoeba Acanthamoeba castellanii. AlamarBlue Assay Reagent® was used to determine the activity against trophozoites of A. castellanii, and cytotoxic using Vero cells. Cysticidal activity was assessed on treated cysts by light microscopy using a Neubauer chamber to quantify cysts and trophozoites. Flavonoids 1, 2, 3 and 4 showed higher trophozoital activity and selectivity indexes than the reference drug chlorhexidine digluconate. In addition, flavonoid 2 showed 100% cysticidal activity at a concentration of 50 μm, lower than those of the reference drug and flavonoid 3 (100 μm). These results suggest that flavonoids 2 and 3 might be used for the development of novel therapeutic approaches against Acanthamoeba infections after satisfactory in vivo evaluations.

Keywords

Acanthamoeba castellaniiamoebicidalchlorhexidine digluconatecysticidalflavonoids
Type
Research Article
Information
Parasitology , Volume 148 , Issue 11 , September 2021 , pp. 1392 - 1400
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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