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Improved specificity of Trypanosoma cruzi identification by polymerase chain reaction using an oligonucleotide derived from the amino-terminal sequence of a Tc24 protein

Published online by Cambridge University Press:  06 April 2009

A. Taibi ,
A. Guevara-Espinoza ,
R. Schöneck ,
B. Yahiaoui  and
A. Ouaissi
A. Taibi
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité INSERM U415, Institut Pasteur, 59019 Lille cédex, France
A. Guevara-Espinoza
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité INSERM U415, Institut Pasteur, 59019 Lille cédex, France
R. Schöneck
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité INSERM U415, Institut Pasteur, 59019 Lille cédex, France
B. Yahiaoui
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité INSERM U415, Institut Pasteur, 59019 Lille cédex, France
A. Ouaissi
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité INSERM U415, Institut Pasteur, 59019 Lille cédex, France
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Summary

In the present study, the diagnostic value of Trypanosoma cruzi recombinant protein (Tc24) was examined. Although antibodies against Tc24 were detected during natural and experimental T. cruzi infections, specificity studies revealed that sera from T. rangeli-infected mice also recognized to some extent Tc24 protein. In addition, sera from Tc24-immunized mice reacted against a 21 kDa polypeptide in T. rangeli extracts. Detailed analysis of the antibody response against 20—40 peptide localized in the Tc24 amino-terminal domain suggests that this sequence is not expressed by T. rangeli 21 kDa antigen. Therefore, the PCR reaction using oligonucleotides corresponding to a 20–26 peptide clearly demonstrated the specificity of the oligoprobes for T. cruzi identification. Positive signals were also found when using blood samples from T. cruzi-infected mice. Taken together, these results suggest that the PCR-based 20–26 assay may be useful in the specific diagnosis of Chagas' disease.

Keywords

Trypanosoma cruziTrypanosoma rangeliTc24 recombinant proteindiagnosisPCR
Type
Research Article
Information
Parasitology , Volume 111 , Issue 5 , December 1995 , pp. 581 - 590
Copyright
Copyright © Cambridge University Press 1995

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