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Implantation of adult Onchocerca gutturosa into laboratory rodents

Published online by Cambridge University Press:  06 April 2009

K. A. El Sinnary ,
A. E. Bianco  and
J. F. Williams
K. A. El Sinnary
Affiliation:
Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1314, USA
A. E. Bianco
Affiliation:
Imperial College of Science, Department of Pure and Applied Biology, Prince Consort Road, London SW7 2BB, UK
J. F. Williams*
Affiliation:
Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824-1314, USA
*
*Reprint requests: Dr J. F. Williams.
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Summary

Adult males and females of Onchocerca gutturosa were implanted into the peritoneal cavity of mice, and their survival was determined at intervals thereafter by post-mortem examination. Ten of 17 animals receiving males dissected free of bovine host tissues contained live parasites at necropsy up to 4 months later. Female worms digested free from connective tissue by collagenase did not survive well, even though they appeared motile and intact before implantation; only 2 were alive 10 days later in 2 of 16 recipients. When males and females still contained within connective tissue capsules were implanted they survived for up to 2 months, and microfilariae were detected transiently in the skin of 2 recipient mice. The results suggest the feasibility of maintaining adult O. gutturosa in rodents by this means, provided exposure of worms to enzymes used to free them from host tissue is avoided.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 1 , August 1987 , pp. 155 - 158
Copyright
Copyright © Cambridge University Press 1987

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