Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-24T17:08:10.822Z Has data issue: false hasContentIssue false
  • English
  • Français

Impairment of growth of Leishmania donovani by Trypanosoma brucei during co-culture

Published online by Cambridge University Press:  06 April 2009

I. Coppens ,
B. H. Ter Kuile  and
F. R. Opperdoes
I. Coppens
Affiliation:
Cell Biology Unit, University of Louvain Medical School and International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
B. H. Ter Kuile
Affiliation:
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
F. R. Opperdoes
Affiliation:
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
Get access Rights & Permissions [Opens in a new window]

Summary

Cells of Leishmania donovani in co-culture with Trypanosoma brucei, were severely affected in their growth, resulting in swelling and subsequent lysis. Similar effects were also observed when Crithidia luciliae or Phytomonas sp. were co-cultured with T. brucei. Direct contact between the cells under investigation and T. brucei was necessary because T. brucei did not hamper the growth of the other trypanosomatids, when separated by a filter with 0.2 μm pore size. Examination of this phenomenon at the ultrastructural level, in a co-culture of L. donovani and T. brucei, suggests that the plasma membrane permeability is increased in the former, as a result of a close cellular contact between the two cell types.

Keywords

Trypanosoma bruceiLeishmania donovani infantumPhytomonasCrithidia luciliaecytolysisinteractions
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 393 - 398
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andrews, N. M. & Webster, P. (1991). Phagolysosomal escape by intracellular pathogens. Parasitology Today 7, 335–40.CrossRefGoogle ScholarPubMed
Baudhuin, P., Evrard, P. & Berthet, J. (1967). Electron microscopic examination of subcellular fractions. I. Preparation of representative samples from suspensions of particles. Journal of Cell Biology 56, 181–91.CrossRefGoogle Scholar
Brun, R. & Schonenberger, M. (1979). Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Tropica 36, 289–92.Google Scholar
Henkart, P. A. (1985). Mechanism of lymphocyte-mediated cytotoxicity. Annual Review of Immunology 3, 31–9.CrossRefGoogle ScholarPubMed
Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17, 208–12.CrossRefGoogle ScholarPubMed
Spurr, A. R. (1969). A low viscosity epoxy resin embedding medium for electronmicroscopy. Journal of Ultrastructure Research 26, 31–43.CrossRefGoogle Scholar
Ter Kuile, B. H. & Opperdoes, F. R. (1991). Chemostat cultures of Leishmania donovani promastigotes and Trypanosoma brucei procyclic trypomastigotes. Molecular and Biochemical Parasitology 45, 171–4.CrossRefGoogle ScholarPubMed
Ter Kuile, B. H. & Opperdoes, F. R. (1992). Comparative physiology of two protozoan parasites Leishmania donovani and Trypanosoma brucei grown in chemostats. Journal of Bacteriology 174, 2929–34.CrossRefGoogle ScholarPubMed
Veldkamp, H. (1986). Continuous Culture in Microbial Physiology and Ecology. Durham: Meadowfield Press.Google Scholar