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Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4

Published online by Cambridge University Press:  17 October 2003

T. G. V. SILVEIRA ,
H. K. TAKAHASHI  and
A. H. STRAUS
T. G. V. SILVEIRA
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil Present address: Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, PR, 87020-900, Brasil.
H. K. TAKAHASHI
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
A. H. STRAUS
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
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Abstract

The immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. The antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. The reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogeneity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. The 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. In contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radio-immunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.

Keywords

Leishmania (Viannia) braziliensismonoclonal antibodyspecific antigensflagellum
Type
Research Article
Information
Parasitology , Volume 127 , Issue 5 , November 2003 , pp. 449 - 456
Copyright
2003 Cambridge University Press

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