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Immunization with SmIg, a novel tegument protein from Schistosoma mansoni, fails to induce protection in mice but reduces liver pathology

Published online by Cambridge University Press:  16 October 2009

J. M. R. PINHO ,
F. C. CARDOSO ,
D. O. LOPES ,
C. S. PINHEIRO ,
M. V. CALIARI ,
F. M. S. OLIVEIRA ,
L. C. LEITE  and
S. C. OLIVEIRA
J. M. R. PINHO
Affiliation:
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
F. C. CARDOSO
Affiliation:
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
D. O. LOPES
Affiliation:
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
C. S. PINHEIRO
Affiliation:
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
M. V. CALIARI
Affiliation:
Department of General Pathology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
F. M. S. OLIVEIRA
Affiliation:
Department of General Pathology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
L. C. LEITE
Affiliation:
Biotechnology Center, Butantan Institute, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil
S. C. OLIVEIRA*
Affiliation:
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil
*
*Corresponding author: Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais Minas Gerais, Belo Horizonte, MG, Brazil. Tel/Fax: +55 31 34092666. E-mail: [email protected]
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Summary

Proteins associated with the schistosome tegument are of great importance for the development of new intervention strategies since they may be exposed on the surface of the parasite. Herein, we have isolated a cDNA clone encoding for the Schistosoma mansoni SmIg and its recombinant protein was tested as a potential vaccine candidate. Initially, its amino acid sequence was analysed by bioinformatics and shown to possess an N-terminal signal peptide, a C-terminal transmembrane helix, 4 glycosylation sites, an immunoglobulin conserved domain and 73% similarity with a hypothetical S. japonicum protein of unknown function. SmIg was produced by E. coli as a recombinant protein (rSmIg) and its protective effectiveness was evaluated against S. mansoni infection with 100 cercariae in a murine model. Mice immunized with rSmIg induced an immune response characterized by dominant IgG1 isotype and significant levels of IFN-γ, TNF-α, IL-10 and IL-4. Although immunogenic, the recombinant vaccine failed to induce worm burden reduction when compared to the infected control group. However, rSmIg-immunized mice had significant reductions of liver granuloma volume and fibrosis content by 31·8% and 49%, respectively. In conclusion, SmIg is a new tegument protein from S. mansoni that plays an important role in reducing pathology induced by parasite infection.

Keywords

SmIgtegument proteinvaccineSchistosoma mansoni
Type
Research Article
Information
Parasitology , Volume 137 , Issue 7 , June 2010 , pp. 1079 - 1088
Copyright
Copyright © Cambridge University Press 2009

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