Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-12-01T22:06:40.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunity to an attenuated variant of Plasmodium berghei: role of some non-specific factors

Published online by Cambridge University Press:  06 April 2009

Seiji waki ,
Shushke Nakazawa ,
Janice Taverne ,
G. A. T. Targett  and
J. H. L. Playfair
Seiji waki
Affiliation:
Department of Parasitology, Gunma University School of Medicine, Maebashi 371, Japan
Shushke Nakazawa
Affiliation:
Department of Parasitology, Gunma University School of Medicine, Maebashi 371, Japan
Janice Taverne
Affiliation:
Department of Immunology, Middlesex Hospital Medical School, London W1P 9PG
G. A. T. Targett
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, London WC1E 7HT
J. H. L. Playfair*
Affiliation:
Department of Immunology, Middlesex Hospital Medical School, London W1P 9PG
*
*Reprint requests: Professor J. H. L. Playfair, Department of Immunology, Middlesex Hospital Medical School, London W1P 9PG.
Get access Rights & Permissions [Opens in a new window]

Extract

Plasmodium berghei XAT, an attenuated variant of lethal P. berghei, causes a resolving infection in Balb/c mice from which they recover in about 3 weeks. The parasitaemia displays an early peak at about 5 days, followed by a steep drop in parasite number associated with the appearance of degenerating forms inside mature erythrocytes; the parasites remaining are inside reticulocytes. By contrast, no degenerating parasites were seen in infections caused by the virulent parent, which was mainly confined to mature erythrocytes. However, P. berghei XAT was no more sensitive to reactive O2 metabolites, generated by alloxan, or to tumour necrosis serum, than its virulent parent. Furthermore, its early drop in parasitaemia was unaffected by silica. The drop still occurred in the absence of T cells, although the infection was then ultimately lethal, and it was not mediated by NK cells since it occurred in nude mice treated with anti-asialo GM1 serum to abolish NK cell activity. However, it was absent in splenectomized mice, in which P. berghei XAT infection was lethal. Thus, the attenuation of P. berghei XAT infection is not due to increased susceptibility to some of the agents thought to cause parasite destruction, but to some other mechanism in which the spleen is involved.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 2 , October 1985 , pp. 263 - 272
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Brinkmann, V., kaufmann, S. H. E., Simon, M. M. & Fischer, H. (1984) Role of macrophages in malaria: 02 metabolite production and phagocytosis by splenic macrophages during lethal Plasmodium berghei and self-limiting Plasmodium yoelii infection in mice. Infection and Immunity 44, 743–6.CrossRefGoogle Scholar
Clark, I. A., Cox, F. E. G. & Allison, A. C. (1977). Protection of mice against Babesia spp. And Plasmodium spp, with killed Corynebacterum parvum. Parasitology 74, 9–18.CrossRefGoogle Scholar
Clark, I. A. & Hunt, N. H. (1983). Evidence for reactive oxygen intermediates causing hemolysis and parasite death in malaria. Infection and Immunity 39, 1–6.CrossRefGoogle ScholarPubMed
Clark, I. A., Virelizier, J.-L., Carswell, E. A. & Wood, P. R. (1981). Possible importance of macrophage-derived mediators in acute malaria. Infection and Immunity 32, 1058–66.CrossRefGoogle ScholarPubMed
Cox, F. E. G. (1983). Differential susceptibility of rodent malaria parasites to nonspecific immunity. Bulletin de la Société de Pathologie Exotique 76, 503–8.Google ScholarPubMed
Dockrell, H. M. & Playfair, J. H. L. (1983). Killing of blood-stage murine malaria parasites by hydrogen peroxide. Infection and Immunity 39, 456–9.CrossRefGoogle ScholarPubMed
Green, S., Dobrjansky, A. & Chiasson, M. A. (1982). Murine tumor necrosis-inducing factor: Purification and effects on myelomonocytic leukemia cells. Journal of the National Cancer Institute 68, 997–1002.Google ScholarPubMed
Grun, J. L. & Weidanz, W. P. (1983). Antibody-independent immunity to reinfection malaria in B-cell-deficient mice. Infection and Immunity 41, 1197–204.CrossRefGoogle ScholarPubMed
Habu, S., Fukui, H., Shimamura, K., Kasai, M., Nagai, Y., Okamura, K. & Tamaoki, N. (1981). In vivo effects of anti-asialo GM1 1. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice. Journal of Immunology 127, 34–8.CrossRefGoogle ScholarPubMed
Haidaris, C. G., Haynes, J. D., Meltzer, M. S. & Allison, A. C. (1983). Serum containing tumor necrosis factor is cytotoxic for the human malaria parasite Plasmodium falciparum. Infection and Immunity 42, 385–93.CrossRefGoogle ScholarPubMed
Jayawardena, A. N., Mogil, R., Murphy, D. B., Burger, D. & Gershon, R. K. (1983). Enhanced expression of H-2 K and H-2 D antigens on reticulocytes infected with Plasmodium yoelii. Nature, London 14, 63–6.Google Scholar
Ockenhouse, C. F. & Shear, H. L. (1984). Oxidative killing of the intraerythrocytic malaria parasite Plasmodium yoelii by activated macrophages. Journal of Immunology 132, 424–31.CrossRefGoogle ScholarPubMed
Playfair, J. H. L. (1982). Immunity to malaria. British Medical Bulletin 38, 153–9.CrossRefGoogle ScholarPubMed
Playfair, J. H. L., De Souza, J. B. & Cottrell, B. J., (1977). Protection of mice against malaria by a killed vaccine: differences in effectiveness against P. Yoelii and P. Berghei. Immunology 33, 507–15.Google ScholarPubMed
Playfair, J. H. L., De Souza, J. B., Dockrell, H. M., Agomo, P. U. & Taverne, J. (1979). Cell-mediated immunity in the liver of mice vaccinated against malaria. Nature, London 282, 731–4.CrossRefGoogle ScholarPubMed
Roberts, D. W. & Weidanz, W. P. (1978). Splenomegaly, enhanced phagocytosis, and anemia are thymus-dependent responses to malaria. Infection and Immunity 20, 728–31.CrossRefGoogle ScholarPubMed
Rzepczyk, C. M. (1982). Probable macrophage origin of the lipopolysaccharide-induced cytostatic effect on intra-erythrocytic malarial parasites (Plasmodium vinckei). Immunology 46, 261–70.Google ScholarPubMed
Shear, H. L., Nussenzweig, R. S. & Bianco, C. (1979). Immune phagocytosis in murine malaria. Journal of Experimental Medicine 149, 1288–98.CrossRefGoogle ScholarPubMed
Skamene, E., Stevenson, M. M. & Lemieux, S. (1983). Murine malaria: dissociation of natural killer (NK) cell activity and resistance to Plasmodium chabaudi. Parasite Immunology 5, 557–65.CrossRefGoogle ScholarPubMed
Suzuki, M., Waki, S., Igarashi, I., Tamura, J., Imanaka, M. & Ishikawa, S. (1980). Host responses induced in mice by a radiation-attenuated Plasmodium berghei (NK65) malaria parasite. International Journal of Nuclear Medicine 7, 141–8.Google ScholarPubMed
Taverne, J., Depledge, P. & Playfair, J. H. L. (1982). Differential sensitivity in vivo of lethal and non-lethal malarial parasites to endotoxin-induced serum factor. Infection and Immunity 37, 927–34.CrossRefGoogle Scholar
Taverne, J., Matthews, N., Depledge, P. & Playfair, J. H. L. (1984). Malarial parasites and tumour cells are killed by the same component of tumour necrosis serum. Clinical and Experimental Immunology 57, 293–300.Google ScholarPubMed
Waki, S., Tamura, J., Imanaka, M., Ishikawa, S. & Suzuki, M. (1982). Plasmodium berghei: isolation and maintenance of an irradiation attenuated strain in the nude mouse. Experimental Parasitology 53, 335–40.CrossRefGoogle ScholarPubMed
Waki, S., Tonome, I. & Suzuki, M. (1983). Plasmodium falciparum: attenuation by irradiation. Experimental Parasitology 56, 339–45.CrossRefGoogle ScholarPubMed
Waki, S., Targett, G. A. T., De Souza, J. B. & Playfair, J. H. L. (1984). Antilymphocyte auto-antibodies in lethal mouse malaria and their absence in an irradiation-induced non-lethal variant. Clinical and Experimental Immunology 55, 245–6.Google Scholar
Wood, P. R. & Clark, I. A. (1982). Apparent irrelevance of NK cells to resolution of infections with Babesia microti and Plasmodium vinckei petteri in mice. Parasite Immunology 4, 319–27.CrossRefGoogle ScholarPubMed
Wozencraft, A. O., Dockrell, H. M., Taverne, J., Targett, G. A. T. & Playfair, J. H. L. (1984). Killing of human malaria parasites by macrophage secretory products. Infection and Immunity 43, 664–9.CrossRefGoogle ScholarPubMed