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Immune expulsion of Trichuris muris from mice during a primary infection: analysis of the components involved

Published online by Cambridge University Press:  06 April 2009

D. Wakelin
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 1QH

Extract

Immune serum accelerated the expulsion of Trichuris muris when transferred into normal mice on days 0 and 3 after infection, but had no effect when the recipient mice had been immunosuppressed by sublethal irradiation or by cortisone treatment. Delaying serum transfer until days 7 and 8 in normal mice failed to accelerate expulsion, although immune mesenteric lymph node cells (MLNC) accelerated expulsion whether transferred early or late in infection.

Expulsion from NIH mice, normally complete by 12 days, was prevented by sublethal irradiation given as late as 9 days after infection, but could be restored by subsequent transfer of immune MLNC or, to a lesser degree, non-immune MLNC. Immune MLNC were unable to restore worm expulsion in mice irradiated before infection.

These results are interpreted as showing that the immune expulsion of T. muris from mice during a primary infection requires the sequential activities of antibody-mediated and lymphoid cell-mediated components.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

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References

REFERENCES

Davies, A. J. S., Doe, A., Cross, A. M. & Elliot, E. V. (1964). Retention of immunological information I. By syngeneic radiation chimaeras. Nature, London 203, 1039–42.CrossRefGoogle ScholarPubMed
Dineen, J. K. & Kelly, J. D. (1973). Expulsion of Nippostrongylus brasiliensis from the intestine of rats: the role of a cellular component derived from bone marrow. International Archives of Allergy and Applied Immunology 45, 759–66.CrossRefGoogle ScholarPubMed
Dineen, J. K., Kelly, J. D. & Love, R. J. (1973). The competence of lymphocytes obtained from immune and non-immune donors to cause expulsion of Nippostrongylus brasiliensis in the rat (DA strain). International Archives of Allergy and Applied Immunology 45, 504–12.CrossRefGoogle ScholarPubMed
Dineen, J. K., Kelly, J. D., Goodrich, B. S. & Smith, I. D. (1974). Expulsion of Nippostrongylus brasiliensis from the small intestine of the rat by prostaglandin-like factors from ram semen. International Archives of Allergy and Applied Immunology 46, 360–74.CrossRefGoogle ScholarPubMed
Dineen, J. K., Ogilvie, B. M. & Kelly, J. D. (1973). Expulsion of Nippostrongylus brasiliensis from the intestine of rats: collaboration between humoral and cellular components of the immune response. Immunology 24, 467–75.Google ScholarPubMed
Harris, G. & Sljivic, V. (1972). The acute effects of ionizing radiation on antibody-producing cells (PFC) in mouse spleen during primary and secondary responses to sheep erythrocytes (SRC). Immunology 23, 147–58.Google ScholarPubMed
Jones, V. E. & Ogilvie, B. M. (1971). Protective immunity to Nippostrongylus brasiliensis: the sequence of events which expels worms from the rat intestine. Immunology 20, 549–61.Google ScholarPubMed
Keller, R. & Keist, R. (1972). Protective immunity to Nippostrongylus brasiliensis in the rat: central role of the lymphocyte in worm expulsion. Immunology 22, 767–73.Google ScholarPubMed
Kelly, J. D., Dineen, J. K. & Love, R. J. (1973). Expulsion of Nippostrongylus brasiliensis from the intestines of rats: evidence for a third component in the rejection mechanism. International Archives of Allergy and Applied Immunology 45, 767–79.CrossRefGoogle ScholarPubMed
Makinodan, T., Nettesheim, P., Morita, T. & Chadwick, C. J. (1967). Synthesis of antibody by spleen cells after exposure to kiloroentgen doses of ionizing radiation. Journal of Cellular Physiology 69, 355–66.CrossRefGoogle ScholarPubMed
Murray, M. (1972). Immediate hypersensitivity effector mechanisms. II. In vivo reactions. In Immunity to Parasitic Animals (ed. Soulsby, E. J. L.), pp. 155–90. New York and London: Academic Press.Google Scholar
Raff, M. C. (1970). Two distinct populations of peripheral lymphocytes in mice distinguish able by immunofluorescence. Immunology 19, 637–50.Google Scholar
Selby, G. R. & Wakelin, D. (1973). Transfer of immunity against Trichuris muris in the mouse by serum and cells. International Journal for Parasitology 3, 717–22.CrossRefGoogle ScholarPubMed
Taliaferro, W. H., Taliaferro, L. G. & Jaroslow, B. N. (1964). Radiation and Immune Mechanisms. New York and London: Academic Press.Google Scholar
Trowell, O. A. (1952). The sensitivity of lymphocytes to ionizing radiation. Journal of Pathology and Bacteriology 64, 687704.CrossRefGoogle Scholar
Wakelin, D. (1967). Acquired immunity to Trichuris muris in the albino laboratory mouse. Parasitology 57, 515–24.CrossRefGoogle ScholarPubMed
Wakelin, D. & Selby, G. R. (1974). Thymus-dependency of the immune response of mice to a primary infection with the nematode Trichuris muris. International Journal for Parasitology (in the Press).CrossRefGoogle Scholar