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Immune complex detection in saliva samples: an innovative proposal for the diagnosis of human strongyloidiasis

Published online by Cambridge University Press:  14 December 2017

L. R. Bosqui ,
A. L. R. Gonçalves ,
M. R. F. Gonçalves-Pires ,
W. R. Pavanelli ,
I. Conchon-Costa ,
J. M. Costa-Cruz  and
I. N. Costa
L. R. Bosqui
Affiliation:
Departamento de Ciências Patológicas, Laboratório de Parasitologia, Universidade Estadual de Londrina, Londrina PR, Brazil
A. L. R. Gonçalves
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia MG, Brazil
M. R. F. Gonçalves-Pires
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia MG, Brazil
W. R. Pavanelli
Affiliation:
Departamento de Ciências Patológicas, Laboratório de Parasitologia, Universidade Estadual de Londrina, Londrina PR, Brazil
I. Conchon-Costa
Affiliation:
Departamento de Ciências Patológicas, Laboratório de Parasitologia, Universidade Estadual de Londrina, Londrina PR, Brazil
J. M. Costa-Cruz
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia MG, Brazil
I. N. Costa*
Affiliation:
Departamento de Ciências Patológicas, Laboratório de Parasitologia, Universidade Estadual de Londrina, Londrina PR, Brazil
*
Author for correspondence: Idessânia Nazareth da Costa, Email: [email protected]
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Abstract

Human strongyloidiasis is caused by helminth Strongyloides stercoralis. It has a worldwide distribution, often neglected and cause of severe morbidity. The parasitological diagnosis is hindered by the low and irregular amount of larvae in feces. The goal of the present study was to detect IgG and IgG immune complex using conventional serum samples and saliva as alternative samples. We collected samples from 60 individuals, namely: group I composed of 30 healthy individuals; and group II composed of 30 individuals eliminating S. stercoralis larvae in feces. We calculated the area under the curve, general index of diagnostic accuracy, Kappa index and determined the correlations between different diagnostic tests. The detection of IgG levels was performed by an immunoenzymatic assay with alkaline extract of S. venezuelensis larvae as antigen. Positivity of anti-S. stercoralis IgG in serum samples from group I was 3·3%, and from group II 93·3%. The detection of immune complex indicated that group I exhibited 3·3% and group II 56·7%. In the saliva samples, IgG detection was 26·7% for group I and 43·3% for group II. Immune complex was detected in 20% of group I, and 30% of group II. IgG immune complex in conventional serum samples and saliva as alternative samples can be considered biomarkers for the diagnosis of active strongyloidiasis.

Keywords

Human strongyloidiasisimmunodiagnosticimmune complexsaliva
Type
Research Article
Information
Parasitology , Volume 145 , Issue 8 , July 2018 , pp. 1090 - 1094
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Copyright
Copyright © Cambridge University Press 2017 

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