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IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis using Taenia crassiceps hydrophobic antigens

Published online by Cambridge University Press:  23 October 2019

Guilherme M. P. Carrara ,
Gabriela B. Silva ,
Lucas S. Faria ,
Daniela S. Nunes ,
Vanessa S. Ribeiro ,
Camila A. Lopes ,
Maria do Rosário F. Gonçalves-Pires ,
Malu M. Santos ,
Isabela P. Borges  and
Álvaro Ferreira-Junior
...Show all authors
Guilherme M. P. Carrara
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Gabriela B. Silva
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Lucas S. Faria
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Daniela S. Nunes
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Vanessa S. Ribeiro
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Camila A. Lopes
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Maria do Rosário F. Gonçalves-Pires
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
Malu M. Santos
Affiliation:
Programa de Pós-Graduação em Sanidade e Produção Animal nos Trópicos, Universidade de Uberaba, Campus Aeroporto. Av. Nenê Sabino, sala 2D05, Uberaba, Minas Gerais38055-500, Brasil
Isabela P. Borges
Affiliation:
Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais38400-902, Brasil
Álvaro Ferreira-Junior
Affiliation:
Programa de Pós-Graduação em Sanidade e Produção Animal nos Trópicos, Universidade de Uberaba, Campus Aeroporto. Av. Nenê Sabino, sala 2D05, Uberaba, Minas Gerais38055-500, Brasil
Veridiana M. R. Ávila
Affiliation:
Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais38400-902, Brasil
Julia M. Costa-Cruz*
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil
*
Author for correspondence: Julia M. Costa-Cruz, E-mail: [email protected]
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Abstract

Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.

Keywords

Egg yolk IgYELISAimmune complexesneurocysticercosisTaenia solium
Type
Research Article
Information
Parasitology , Volume 147 , Issue 2 , February 2020 , pp. 240 - 247
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Copyright
Copyright © Cambridge University Press 2019

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