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Identification of the agent causing visceral leishmaniasis in Uzbeki and Tajiki foci by analysing parasite DNA extracted from patients' Giemsa-stained tissue preparations

Published online by Cambridge University Press:  24 June 2009

M. Z. ALAM ,
D. A. KOVALENKO ,
K. KUHLS ,
R. M. NASYROVA ,
V. I. PONOMAREVA ,
A. A. FATULLAEVA ,
S. A. RAZAKOV ,
L. F. SCHNUR  and
G. SCHÖNIAN
M. Z. ALAM*
Affiliation:
Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Dorotheenstrasse 96, D-10098 Berlin, Germany Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
D. A. KOVALENKO
Affiliation:
Isaev Institute of Medical Parasitology, Samarkand, Uzbekistan
K. KUHLS
Affiliation:
Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Dorotheenstrasse 96, D-10098 Berlin, Germany
R. M. NASYROVA
Affiliation:
Isaev Institute of Medical Parasitology, Samarkand, Uzbekistan
V. I. PONOMAREVA
Affiliation:
Isaev Institute of Medical Parasitology, Samarkand, Uzbekistan
A. A. FATULLAEVA
Affiliation:
Isaev Institute of Medical Parasitology, Samarkand, Uzbekistan
S. A. RAZAKOV
Affiliation:
Isaev Institute of Medical Parasitology, Samarkand, Uzbekistan
L. F. SCHNUR
Affiliation:
Department of Parasitology, Hebrew University–Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
G. SCHÖNIAN
Affiliation:
Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Dorotheenstrasse 96, D-10098 Berlin, Germany
*
*Corresponding author: Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Dorotheenstrasse 96, D-10098 Berlin, Germany. Tel: 0049 30 450524058. Fax: 0049 30 450524902. E-mail: [email protected]
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Summary

Our present study is the first attempt to characterize Leishmania parasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of Giemsa-stained bone marrow aspirates from 13 cases of VL. L. infantum was shown to cause VL currently occurring in this area. MLMT applying 14 microsatellite markers, previously shown to be polymorphic for strains of the L. donovani complex, revealed that microsatellite profiles of parasites causing human VL in the Namangan and Jizzakh regions in Uzbekistan, and Penjikent region in Tajikistan, basically coincide with those of strains of L. infantum MON-1. Furthermore, these parasites were assigned to a distinct cluster genetically clearly separated from the populations of L. infantum MON-1 from Europe, the Middle East and North Africa. The existence of a genetically homogeneous but distinct group of L. infantum MON-1 indicates that the parasites circulating in the Uzbeki and Tajiki foci studied have been restricted there for a long time rather than having been recently introduced from elsewhere by human or animal reservoir migration.

Keywords

visceral leishmaniasisLeishmania infantuminternal transcribed spacer 1microsatellite analysisUzbekistan
Type
Research Article
Information
Parasitology , Volume 136 , Issue 9 , August 2009 , pp. 981 - 986
Copyright
Copyright © Cambridge University Press 2009

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