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Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling

Published online by Cambridge University Press:  08 January 2020

Fatalmoudou Tandina ,
Sirama Niare ,
Lionel Almeras ,
Bernard Davoust ,
Ogobara K Doumbo ,
Didier Raoult ,
Philippe Parola  and
Maureen Laroche Open the ORCID record for Maureen Laroche [Opens in a new window]
Fatalmoudou Tandina
Affiliation:
Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France Malaria Research and Training Center, DEAP/FMOS, UMI 3189, University of Science, Techniques and Technology, Bamako, Mali IHU-Méditerranée Infection, Marseille, France
Sirama Niare
Affiliation:
Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France Malaria Research and Training Center, DEAP/FMOS, UMI 3189, University of Science, Techniques and Technology, Bamako, Mali IHU-Méditerranée Infection, Marseille, France
Lionel Almeras
Affiliation:
Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France IHU-Méditerranée Infection, Marseille, France Unité de Parasitologie et d'Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France
Bernard Davoust
Affiliation:
IHU-Méditerranée Infection, Marseille, France Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
Ogobara K Doumbo
Affiliation:
Malaria Research and Training Center, DEAP/FMOS, UMI 3189, University of Science, Techniques and Technology, Bamako, Mali
Didier Raoult
Affiliation:
IHU-Méditerranée Infection, Marseille, France Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
Philippe Parola
Affiliation:
Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France IHU-Méditerranée Infection, Marseille, France
Maureen Laroche*
Affiliation:
Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France IHU-Méditerranée Infection, Marseille, France
*
Author for correspondence: Maureen Laroche, E-mail: [email protected]
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Abstract

Background

The accurate and rapid identification of mosquito blood meals is critical to study the interactions between vectors and vertebrate hosts and, subsequently, to develop vector control strategies. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has been shown to be a reliable and effective tool for identifying single blood meals from mosquitoes.

Methods

In this study, we developed MALDI-TOF MS profiling protocols to identify Anopheles gambiae Giles, Anopheles coluzzii and Aedes albopictus mosquitoes’ mixed blood meals and the last of successive blood meals. The mosquitoes were either successively artificially fed with distinct host bloods or engorged with mixed bloods from distinct vertebrate hosts, such as humans, sheep and dogs.

Results

Blind test analyses revealed a correct identification of mixed blood meals from mosquitoes using MALDI-TOF MS profiling. The 353 MS spectra from mixed blood meals were identified using log score values >1.8. All MS spectra (n = 244) obtained from mosquitoes' successive blood meals were reproducible and specific to the last blood meal, suggesting that the previous blood meals do not have an impact on the identification of the last one.

Conclusion

MALDI-TOF MS profiling approach appears to be an effective and robust technique to identify the last and mixed blood meals during medical entomological surveys.

Keywords

AedesAnophelesblood mealsentomological surveyMALDI-TOF MS profiling
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 329 - 339
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Copyright
Copyright © Cambridge University Press 2019

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Footnotes

*

These authors contributed equally to this work.

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