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Identification of common surface antigens among Babesia bigemina isolates by using monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

J. V. Figueroa ,
G. M. Buening ,
D. A. Kinden  and
T. J. Green
J. V. Figueroa
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
G. M. Buening
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
D. A. Kinden
Affiliation:
Veterinary PathologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
T. J. Green
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
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Summary

A group of monoclonal antibodies (MAb) was used to immunochemically characterize Babesia bigemina surface components. Two surface reactive MAb by an IFAT test were subsequently shown to bind to epitopes on the external surface of the parasite plasma membrane as evidenced by immunoelectron microscopy. Parasite components with relative sizes of 68, 62, 60, 58, 56, 54, 51, 49, 48, 47, 43, 36 kDa were identified with the group of MAb in at least 6 geographically different B. bigemina isolates. The antigenic components were demonstrated to be species-specific and apparently predominant. Both polyclonal antibodies (immune bovine serum) and highly diluted MAb specifically reacted with such bands producing a strong signal in Western blots.

Keywords

Babesia bigeminasurface antigensmonoclonal antibodiesimmunoblottingimmunoprecipitationimmunoelectron microscopy
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 161 - 175
Copyright
Copyright © Cambridge University Press 1990

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