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Identification of a new Schistosoma mansoni SMYB1 partner: putative roles in RNA metabolism

Published online by Cambridge University Press:  14 May 2013

ELIZÂNGELA A. ROCHA ,
ANALINA F. VALADÃO ,
CÍNTIA M. REZENDE ,
SILVIA REGINA COSTA DIAS ,
ANDRÉA M. MACEDO ,
CARLOS R. MACHADO ,
MARCELO R. FANTAPPIÉ ,
FRANKLIN D. RUMJANEK ,
ALFREDO M. GOES  and
DAWIDSON A. GOMES
...Show all authors
ELIZÂNGELA A. ROCHA*
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
ANALINA F. VALADÃO
Affiliation:
Instituto Metropolitano de Ensino Superior (IMES), Univaço, Ipatinga, MG, Brazil
CÍNTIA M. REZENDE
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
SILVIA REGINA COSTA DIAS
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
ANDRÉA M. MACEDO
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
CARLOS R. MACHADO
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
MARCELO R. FANTAPPIÉ
Affiliation:
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
FRANKLIN D. RUMJANEK
Affiliation:
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
ALFREDO M. GOES
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
DAWIDSON A. GOMES
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
PHILIP T. LOVERDE
Affiliation:
Departments of Biochemistry and Pathology, University of Texas Health Science Center, San Antonio, Texas, USA
MARCELA G. DRUMMOND
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
GLÓRIA R. FRANCO*
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
*
*Corresponding author: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais – UFMG, Avenida Antônio Carlos 6627, Belo Horizonte, MG, 31270-010, Brazil. E-mail: [email protected]
*Corresponding author: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais – UFMG, Avenida Antônio Carlos 6627, Belo Horizonte, MG, 31270-010, Brazil. E-mail: [email protected]
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Summary

SMYB1 is a Schistosoma mansoni protein highly similar to members of the Y-box binding protein family. Similar to other homologues, SMYB1 is able to bind double- and single-stranded DNA, as well as RNA molecules. The characterization of proteins involved in the regulation of gene expression in S. mansoni is of great importance for the understanding of molecular events that control morphological and physiological changes in this parasite. Here we demonstrate that SMYB1 is located in the cytoplasm of cells from different life-cycle stages of S. mansoni, suggesting that this protein is probably acting in mRNA metabolism in the cytoplasm and corroborating previous findings from our group that showed its ability to bind RNA. Protein–protein interactions are important events in all biological processes, since most proteins execute their functions through large supramolecular structures. Yeast two-hybrid screenings using SMYB1 as bait identified a partner in S. mansoni similar to the SmD3 protein of Drosophila melanogaster (SmRNP), which is important in the assembly of small nuclear ribonucleoprotein complexes. Also, pull-down assays were conducted using immobilized GST-SMYB1 proteins and confirmed the SMYB1-SmRNP interaction. The interaction of SMYB1 with a protein involved in mRNA processing suggests that it may act in processes such as turnover, transport and stabilization of RNA molecules.

Keywords

Schistosoma mansonitwo-hybridSMYB1RNA metabolism
Type
Research Article
Information
Parasitology , Volume 140 , Issue 9 , August 2013 , pp. 1085 - 1095
Copyright
Copyright © Cambridge University Press 2013 

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