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Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula

Published online by Cambridge University Press:  06 April 2009

D. W. Dunne  and
Q. D. Bickle
D. W. Dunne
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans
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Summary

Antisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction of Schistosoma mansoni SEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to by S. mansoni-infected mouse sera, and has been designated K3 (Kappa 3). The rabbit antisera were used to partially characterize antigen K3 as having Mr in the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3 serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3 serum also reacted with the surface of S. bovis, S. haematobium and to a lesser extent S. japonicum schistosomula.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 255 - 268
Copyright
Copyright © Cambridge University Press 1987

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