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A hybrid gene to express protein epitopes from both sporozoite and merozoite surface antigens of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

A. A. Holder ,
M. J. Lockyer  and
G. W. Hardy
A. A. Holder
Affiliation:
Departments ofWellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
M. J. Lockyer
Affiliation:
Departments ofWellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
G. W. Hardy
Affiliation:
Departments of Molecular Biology and Medicinal Chemistry
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Summary

The DNA coding for parts of the repetitive amino acid sequence of the Plasmodium falciparum circumsporozoite protein has been spliced to a sequence encoding part of the precursor to the major merozoite surface antigens, to produce a hybrid gene. Expression in Escherichia coli produces a protein with antigenic determinants from both malaria proteins. Antibodies raised against the expressed material react with both a peptide derived from the circumsporozoite repeat sequence, and the merozoite surface molecule. Hybrid molecules of this type may be the basis of a malaria vaccine.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 3 , December 1988 , pp. 373 - 382
Copyright
Copyright © Cambridge University Press 1988

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