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Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus

Published online by Cambridge University Press:  30 April 2003

E. A. MACINTYRE ,
C. G. EARNHART  and
S. L. KAATTARI
E. A. MACINTYRE
Affiliation:
Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062–1346, USA
C. G. EARNHART
Affiliation:
Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062–1346, USA
S. L. KAATTARI
Affiliation:
Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062–1346, USA
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Abstract

Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30–45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS–PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.

Keywords

Perkinsus marinusproteaseCrassostrea sp.differentiationin vitro culture
Type
Research Article
Information
Parasitology , Volume 126 , Issue 4 , April 2003 , pp. 293 - 302
Copyright
© 2003 Cambridge University Press

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