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Histopathologic evaluation of experimental murine neurocysticercosis after treatment with albendazole/nitazoxanide combination

Published online by Cambridge University Press:  01 April 2020

Guilherme A. Sampaio Open the ORCID record for Guilherme A. Sampaio [Opens in a new window] ,
Larissa V. Zago Open the ORCID record for Larissa V. Zago [Opens in a new window] ,
Nayana F. de Lima Open the ORCID record for Nayana F. de Lima [Opens in a new window] ,
Guaraciara de A. Picanço Open the ORCID record for Guaraciara de A. Picanço [Opens in a new window] ,
Ruy de S. Lino Júnior Open the ORCID record for Ruy de S. Lino Júnior [Opens in a new window]  and
Marina C. Vinaud Open the ORCID record for Marina C. Vinaud [Opens in a new window]
Guilherme A. Sampaio
Affiliation:
Medicine School, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Larissa V. Zago
Affiliation:
Medicine School, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Nayana F. de Lima
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Guaraciara de A. Picanço
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Ruy de S. Lino Júnior
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Marina C. Vinaud*
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
*
Author for correspondence: Marina Clare Vinaud, E-mail: [email protected]
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Abstract

Neurocysticercosis (NCC) is the most common helminthic brain infection related to epilepsy. Only albendazole (ABZ) and praziquantel are used in its treatment. The development of new therapeutics has been encouraged. Taenia crassiceps cysticerci intracranial infection is the experimental model used in NCC studies. This study evaluated the histopathology of the brains of BALB/c mice experimentally infected with T. crassiceps cysticerci after the treatment with the ABZ/nitazoxanide (NTZ) combination. Thirty days after the inoculation the mice received an oral single dose of the ABZ/NTZ combination (40 mg kg−1 each). The control groups were treated with: NaCl 0.9%; ABZ or NTZ. The histopathologic evaluation of the brains was performed 24 h after treatment. The ABZ treatment induced discrete mononuclear inflammatory infiltration, meningitis, gliosis, hyperaemia and hippocampus compression; moderate ependimitis and oedema. The NTZ treatment induced accentuated inflammatory infiltration, foamy macrophages, ependimitis, choroiditis, gliosis and hyperaemia and moderate oedema. The ABZ/NTZ combination treatment induced a significant decrease in the polymorphonuclear inflammatory infiltration, ependimitis, choroiditis, gliosis, hyperaemia and ventriculomegaly in comparison with the other groups. The cysticerci showed destruction of the tegument not observed in other groups. The ABZ/NTZ combination is efficient as the parasite showed signs of destruction and lower damage to the host's tissue.

Keywords

Albendazoleanthelminthic drugsexperimental neurocysticercosisnitazoxanidepathologic evaluationTaenia crassiceps
Type
Research Article
Information
Parasitology , Volume 147 , Issue 7 , June 2020 , pp. 822 - 827
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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