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Gilthead seabream (Sparus aurata L.) innate defence against the parasite Enteromyxum leei (Myxozoa)

Published online by Cambridge University Press:  21 September 2005

A. CUESTA ,
P. MUÑOZ ,
A. RODRÍGUEZ ,
I. SALINAS ,
A. SITJÀ-BOBADILLA ,
P. ÁLVAREZ-PELLITERO ,
M. A. ESTEBAN  and
J. MESEGUER
A. CUESTA
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
P. MUÑOZ
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
A. RODRÍGUEZ
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
I. SALINAS
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
A. SITJÀ-BOBADILLA
Affiliation:
Instituto de Acuicultura Torre de la Sal (CSIC), 12595 Ribera de Cabanes, Castellón, Spain
P. ÁLVAREZ-PELLITERO
Affiliation:
Instituto de Acuicultura Torre de la Sal (CSIC), 12595 Ribera de Cabanes, Castellón, Spain
M. A. ESTEBAN
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
J. MESEGUER
Affiliation:
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
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Abstract

The humoral innate immune response of gilthead seabream (Sparus aurata L.) against the myxozoan Enteromyxum leei has been studied. At 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mRNA expression of pro-inflammatory cytokines (IL-1β and TNFα) in head-kidney. The parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62·5%) at the last sampling time (108 days). Recipient fish started to die after 74 days of cohabitation. In general, alternative complement activity was higher whereas the peroxidase level was lower in recipient fish than in controls. Moreover, IL-1β mRNA expression increased while the TNFα gene expression decreased in recipient fish. These data demonstrate the involvement of complement activity in the defence mechanisms of the gilthead seabream against the myxosporean E. leei. Within the recipient fish group, few differences were observed in the studied immune parameters between E. leei-parasitized and non-parasitized recipient fish. Parasitological and immunological implications of E. leei infections in Mediterranean fish farms are discussed.

Keywords

Enteromyxum leeiMyxozoaparasitesinnate immunitycomplementcytokinesgilthead seabream (Sparus aurata L.)teleosts
Type
Research Article
Information
Parasitology , Volume 132 , Issue 1 , January 2006 , pp. 95 - 104
Copyright
© 2005 Cambridge University Press

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