Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T22:40:01.576Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotyping of human cystic echinococcosis in Xinjiang, PR China

Published online by Cambridge University Press:  12 July 2006

J. M. BART ,
M. ABDUKADER ,
Y. L. ZHANG ,
R. Y. LIN ,
Y. H. WANG ,
M. NAKAO ,
A. ITO ,
P. S. CRAIG ,
R. PIARROUX  and
D. A. VUITTON
...Show all authors
J. M. BART
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China SERF Unit, Faculty of Medicine, University of Franche-Comté, Besancon 25000, France
M. ABDUKADER
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China
Y. L. ZHANG
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China
R. Y. LIN
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China
Y. H. WANG
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China
M. NAKAO
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan
A. ITO
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan
P. S. CRAIG
Affiliation:
Cestode Zoonoses Research Group, Biomedical Sciences Research Institute and School of Environment and Life Sciences, University of Salford, Greater Manchester M54WT, UK
R. PIARROUX
Affiliation:
SERF Unit, Faculty of Medicine, University of Franche-Comté, Besancon 25000, France
D. A. VUITTON
Affiliation:
SERF Unit, Faculty of Medicine, University of Franche-Comté, Besancon 25000, France
H. WEN
Affiliation:
First Teaching Hospital, Xinjiang Medical University, Urumqi 830000, PR China
Get access Rights & Permissions [Opens in a new window]

Abstract

The Xinjiang Uygur Autonomous Region, multi-ethnic province in northwestern China, is one of the most important foci of human cystic echinococcosis (CE) in the world. Two Echinococcus granulosus genotypes (G1 and G6) are known to infect the intermediate hosts in this area but, to date, the source of the human infection remains unclear. The current study aimed to genetically analyse 67 hydatid cysts removed from 47 CE patients for which epidemiological, clinical and serological data were also recorded. Mitochondrial cox 1 gene sequencing suggested that the E. granulosus G1 genotype is the major source of infection (45/47 CE patients). Nevertheless, for the first time in China, 2 patients were found with hydatid cysts of the G6 genotype. In addition, 45 E. granulosus gravid tapeworms, isolated from 13 dogs, were genotyped. The majority of adult worms (42/45) exhibited the G1 genotype, whereas 3 adult tapeworms with the G6 genotype were found in one dog, that also harboured E. granulosus tapeworms of the G1 genotype. This sympatric occurrence of G1 and G6 genotypes of E. granulosus, not only in the same area but also in the same definitive host, raises the interesting question of putative genetic recombination between these E. granulosus genotypes.

Keywords

Echinococcus granulosusgenotypeXinjianghumandog
Type
Research Article
Information
Parasitology , Volume 133 , Issue 5 , November 2006 , pp. 571 - 579
Copyright
2006 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bardonnet, K., Piarroux, R., Dia, L., Schneegans, F., Beurdeley, A., Godot, V. and Vuitton, D. A. ( 2002). Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in Mauritania: occurrence of the ‘camel’ strain and human cystic echinococcosis. Transaction of the Royal Society of Tropical Medicine and Hygiene 96, 383–386.CrossRefGoogle Scholar
Bardonnet, K., Benchikh-Elfegoun, M. C., Bart, J. M., Harraga, S., Hannache, N., Haddad, S., Dumon, H., Vuitton, D. A. and Piarroux, R. ( 2003). Cystic echinococcosis in Algeria: cattle act as reservoir of a sheep strain and may contribute to human contamination. Veterinary Parasitology 116, 35–44.CrossRefGoogle Scholar
Bart, J. M., Bardonnet, K., Benchikh-Elfegoun, M. C., Dumon, H., Dia, L., Vuitton, D. A. and Piarroux, R. ( 2004). Echinococcus granulosus strain typing in North Africa: comparison of eight nuclear and mitochondrial DNA fragments. Parasitology 128, 229–234.CrossRefGoogle Scholar
Bart, J. M., Morariu, S., Knapp, J., Ilie, M. S., Pitulescu, M., Anghel, A., Cosoroaba, I. and Piarroux, R. ( 2006). Genetic epidemiology of Echinococcus granulosus in Romania. Parasitology Research 98, 130–137.CrossRefGoogle Scholar
Cabrera, M., Canova, S., Rosenzvit, M. and Guarnera, E. ( 2002). Identification of Echinococcus granulosus eggs. Diagnostic Microbiology Infectious Diseases 44, 29–34.CrossRefGoogle Scholar
Chai, J. J. ( 1993). Brief overview of the Xinjiang Uygur Autonomous Region, the People's Republic of China. In Compendium on Cystic Echinococcosis with Special Reference to the Xinjiang Uygur Autonomous region, the People's Republic of China ( ed. Andersen, F. L., Chai, J. J. and Liu, F. J.), pp. 16–20. Provo: Brigham Young University Print Service.
Chai, J. J. ( 1995). Epidemiological studies on cystic echinococcosis in China – a review. Biomedical Environment Sciences 8, 122–136.Google Scholar
Chi, P., Zhang, W. and Zhang, Z. ( 1990). Cystic echinococcosis in the Xinjiang Uygur Autonomous region, the People's Republic of China. 1. Demographic and epidemiological data. Tropical Medicine and Parasitology 41, 157–162.Google Scholar
Craig, P. S. ( 2004). Epidemiology of echinococcosis in China. Southeast Asian Journal of Tropical Medicine and Public Health 35 (Suppl. 1), 158–169.Google Scholar
Craig, P. S., Deshan, L. and Zhaoxun, D. ( 1991). Hydatid disease in China. Parasitology Today 7, 46–50.CrossRefGoogle Scholar
Daeki, A. O., Craig, P. S. and Shambesh, M. K. ( 2000). IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Annals of Tropical Medicine Parasitology 94, 319–328.CrossRefGoogle Scholar
Dinkel, A., Njoroge, E. M., Zimmermann, A., Walz, M., Zeyhle, E., Elmahdi, I. E., Mackenstedt, U. and Romig, T. ( 2004). A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. International Journal for Parasitology 34, 645–653.CrossRefGoogle Scholar
Guarnera, E. A., Parra, A., Kamenetzky, L., Garcia, G. and Gutierrez, A. ( 2004). Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Tropica 92, 153–159.CrossRefGoogle Scholar
Haag, K. L., Ayala, F. J., Kamenetzky, L., Gutierrez, A. M. and Rosenzvit, M. ( 2004). Livestock trade history, geography, and parasite strains: the mitochondrial genetic structure of Echinococcus granulosus in Argentina. Journal of Parasitology 90, 234–239.CrossRefGoogle Scholar
Hall, T. A. ( 1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41, 95–98.Google Scholar
Harandi, M. F., Hobbs, R. P., Adams, P. J., Mobedi, I., Morgan-Ryan, U. M. and Thompson, R. C. ( 2002). Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 125, 367–373.Google Scholar
Kamenetzky, L., Gutierrez, A. M., Canova, S. G., Haag, K. L., Guarnera, E. A., Parra, A., Garcia, G. E. and Rosenzvit, M. C. ( 2002). Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infection, Genetics and Evolution 2, 129–136.CrossRefGoogle Scholar
Li, X. L., Zhang, W. L., Qu, Q., Jiao, W. and Chai, J. J. ( 1992). An analysis of DNA hybridization of protoscoleces of E. granulosus from different sources in Xinjiang. Endemic Disease Bulletin 7 (Suppl.), 125–132 (in Chinese).Google Scholar
Liu, F., Che, X. and Chang, Q. ( 1993). Prevalence of hydatid cysts in livestock in the Xinjiang Uygur Autonomous region, the People's Republic of China. In Compendium on Cystic Echinococcosis with Special Reference to the Xinjiang Uygur Autonomous Region, the People's Republic of China ( ed. Andersen, F. L., Chai, J. J. and Liu, F. J.), pp. 177–189. Provo: Brigham Young University Print Service.
Mamuti, W., Yamasaki, H., Sako, Y., Nakao, M., Xiao, N., Nakaya, K., Sato, N., Vuitton, D. A., Piarroux, R., Lightowlers, M. W., Craig, P. S. and Ito, A. ( 2004). Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis. Journal of Clinical Microbiology 42, 1082–1088.CrossRefGoogle Scholar
McManus, D. P., Ding, X. and Bowles, J. ( 1994). A molecular genetic survey indicates the presence of a single, homogeneous strain of E. granulosus on north-western China. Acta Tropica 56, 7–14.Google Scholar
M'rad, S., Filisetti, D., Oudni, M., Mekki, M., Belguith, M., Nouri, A., Sayadi, T., Lahmar, S., Candolfi, E., Azaiez, R., Mezhoud, H. and Babba, H. ( 2005). Molecular evidence of ovine (G1) and camel (G6) strains of Echinococcus granulosus in Tunisia and putative role of cattle in human contamination. Veterinary Parasitology 129, 267–272.CrossRefGoogle Scholar
Rice, P., Longden, I. and Bleasby, A. ( 2000). EMBOSS: the European molecular biology open software suite. Trends in Genetics 16, 276–277.CrossRefGoogle Scholar
Wachira, T. M., Bowles, J., Zeyhle, E. and McManus, D. P. ( 1993). Molecular examination of the sympatry and distribution of sheep and camel strains of Echinococcus granulosus in Kenya. American Journal of Tropical Medicine and Hygiene 48, 473–479.CrossRefGoogle Scholar
Wang, Y., He, T., Wen, X., Li, T., Waili, T. T., Zhang, W., Zhou, H., Zheng, H., Wen, H., Davaadorj, N., Gambolt, L., Mukhar, T., Rogan, M. T. and Craig, P. S. ( 2005). Human cystic echinococcosis in two Mongolian communities in Hobukesar (China) and Bulgan (Mongolia). Transactions of the Royal Society of Tropical Medicine and Hygiene 99, 692–698.CrossRefGoogle Scholar
Wang, Y. H., Rogan, M. T., Vuitton, D. A., Wen, H., Bartholomot, B., Macpherson, C. N., Zou, P. F., Ding, Z. X., Zhou, H. X., Zhang, X. F., Luo, J., Xiong, H. B., Fu, Y., McVie, A., Giraudoux, P., Yang, W. G. and Craig, P. S. ( 2001). Cystic echinococcosis in semi-nomadic pastoral communities in northwest China. Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 153–158.CrossRefGoogle Scholar
Wen, H. and Yang, W. ( 1997). Public health importance of cystic echinococcosis in China. Acta Tropica 67, 133–145.CrossRefGoogle Scholar
WHO-OIE. ( 2001). Echinococcosis in humans: clinical aspects, diagnosis and treatment. In Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern ( ed. Eckert, J., Gemmel, M. A., Meslin, F.-X. and Pawlowski, Z. S.), WHO, Geneva.
Zhang, L. H., Chai, J. J., Jiao, W., Osman, Y. and McManus, D. P. ( 1998). Mitochondrial genomic markers confirm the presence of the camel strain (G6 genotype) of Echinococcus granulosus in north-western China. Parasitology 116, 29–33.CrossRefGoogle Scholar
Zhang, L. H., Joshi, D. D. and McManus, D. P. ( 2000). Three genotypes of Echinococcus granulosus identified in Nepal using mitochondrial DNA markers. Transaction of the Royal Society of Tropical Medicine and Hygiene 94, 258–260.CrossRefGoogle Scholar