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Free-living amoebae and their relationship to air quality in hospital environments: characterization of Acanthamoeba spp. obtained from air-conditioning systems

Published online by Cambridge University Press:  18 March 2020

Débora Borgert Wopereis ,
Maria Luiza Bazzo ,
Jefferson Peres de Macedo ,
Fabiana Casara ,
Lisléia Golfeto ,
Eduardo Venancio ,
Jaquelline Germano de Oliveira ,
Marilise Brittes Rott  and
Karin Silva Caumo Open the ORCID record for Karin Silva Caumo [Opens in a new window]
Débora Borgert Wopereis
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes e Oportunistas, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil
Maria Luiza Bazzo
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Biologia Molecular, Sorologia e Micobactérias, CEP 88040-900, Florianópolis, Santa Catarina, Brazil
Jefferson Peres de Macedo
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes e Oportunistas, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil
Fabiana Casara
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes e Oportunistas, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil
Lisléia Golfeto
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Biologia Molecular, Sorologia e Micobactérias, CEP 88040-900, Florianópolis, Santa Catarina, Brazil
Eduardo Venancio
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Biologia Molecular, Sorologia e Micobactérias, CEP 88040-900, Florianópolis, Santa Catarina, Brazil
Jaquelline Germano de Oliveira
Affiliation:
Fundação Oswaldo Cruz, Centro de Pesquisas René Rachou, Laboratório de Imunologia Celular e Molecular, CEP: 30.190-002, Belo Horizonte, Minas Gerais, Brazil
Marilise Brittes Rott
Affiliation:
Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde. Departamento de Microbiologia, Imunologia e Parasitologia, CEP 900035-190, Porto Alegre, Rio Grande do Sul, Brazil.
Karin Silva Caumo*
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes e Oportunistas, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil
*
Author for correspondence: Karin Silva Caumo, E-mail: [email protected]
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Abstract

Free-living amoebae (FLA) are widely dispersed in the environment, can cause opportunistic and non-opportunistic infections in humans and other animals. The aim of the present study was characterize FLA obtained from air-conditioners of a public hospital in the city of Florianópolis, SC, Brazil. Fifty-four dust samples were collected of air conditioners, and were inoculated on 1.5% non-nutrient agar, overlaid with layers of Escherichia coli. Subsequently the isolates were axenised in PYG growth medium. The morphological and molecular characterization of the isolates was performed, as well as the tolerance (physiological) assays were used to evaluate the pathogenic potential. The results revealed the presence of FLA in 42 (77.8%) of the collected samples. Of these, 39 (92.9%) axenic isolates of FLA were obtained for morphological and genotypic studies. All the isolates characterized belong to the genus Acanthamoeba. Nineteen (48.7%) isolates belong to the genotype T4, 16 (41.0%) to the T5 genotype and 4 (10.3%) to genotype T11. Seven (18.0%) isolates were considered potentially pathogenic in tolerance assays. These findings require attention, considering the isolation environment and immunocompromised characteristics of many hospitalized patients.

Keywords

Acanthamoebaair-conditionersfree-living amoebaehospital
Type
Research Article
Information
Parasitology , Volume 147 , Issue 7 , June 2020 , pp. 782 - 790
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Afonso, MSM, Tripple, AFV, Souza, ACS, Prado, MA and Anders, PS (2004) A qualidade do ar em ambientes hospitalares climatizados e sua influência na ocorrência de infecções. Revista Eletrônica de Enfermagem 6, 181–188.Google Scholar
Al-Herrawy, A, Bahgat, M, Mohammed, A, Ashour, A and Hikal, W (2013) Morpho-physiological and biochemical criteria of Acanthamoeba spp. isolated from the Egyptian aquatic environment. Iranian Journal of Parasitology 8, 302–312.Google ScholarPubMed
Alves, DSMM, Moraes, AS, Nitz, N, Oliveira, MGC, Hecht, MM, Gurguel-Gonçalves, R and Cuba, CAC (2012) Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil. Experimental Parasitology 131, 239–244.CrossRefGoogle Scholar
Astorga, B, Lorenzo-Morales, J, Martín-Navarro, CM, Alarcón, V, Moreno, J, González, AC, Navarrete, E, Piñero, JE and Valladares, B (2011) Acanthamoeba belonging to T3, T4, and T11: genotypes isolated from air-conditioning units in Santiago, Chile. Journal of Eukaryotic Microbiology 58, 542–544.CrossRefGoogle ScholarPubMed
Balczun, C and Scheid, PL (2017) Free-living amoebae as hosts for and vectors of intracellular microorganisms with public health significance. Viruses 9, 1–18.CrossRefGoogle ScholarPubMed
Booton, GC, Visvesvara, GS, Byers, TJ, Kelly, DJ and Fuerst, PA (2005) Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. Journal of clinical microbiology 43, 1689–1693.CrossRefGoogle ScholarPubMed
Carlesso, AM, Artuso, GL, Caumo, K and Rott, MB (2010) Potentially pathogenic Acanthamoeba isolated from a hospital in Brazil. Current Microbiology 60, 185–190.CrossRefGoogle ScholarPubMed
Caumo, K, Frasson, AP, Pens, CJ, Panatieri, LF, Frazzon, APG and Rott, MB (2009) Potentially pathogenic Acanthamoeba in swimming pools: a survey in the southern Brazilian city of Porto Alegre. Annals of Tropical Medicine & Parasitology 103, 477–485.CrossRefGoogle ScholarPubMed
Chan, LL, Mak, JW, Low, YT, Koh, TT, Ithoib, I and Mohamed, SM (2011) Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia. Acta Tropica 117, 23–30.CrossRefGoogle ScholarPubMed
Corsaro, D, Walochnik, J, Köhsler, M and Rott, MB (2015) Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19). Parasitology Research 114, 2481–2490.Google Scholar
Corsaro, D, Köhsler, M, Di Filippo, MM, Venditti, D, Monno, R, Di Cave, D, Berilli, F and Walochnik, J (2017) Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny. Parasitology Research 116, 1273–1284.CrossRefGoogle ScholarPubMed
Costa, AO, Castro, EA, Ferreira, GA, Furst, C, Crozeta, MA and Thomaz-Soccol, V (2010) Characterization of Acanthamoeba isolates from dust of a public hospital in curitiba, paraná, Brazil. Journal of Eukaryotic Microbiology 57, 70–75.CrossRefGoogle ScholarPubMed
Fuerst, PA, Booton, GC and Crary, M (2015) Phylogenetic analysis and the evolution of the 18S rRNA gene typing system of Acanthamoeba. Journal of Eukaryotic Microbiology 62, 69–84.CrossRefGoogle ScholarPubMed
Geisen, S, Fiore-Donno, AM, Walochnik, J and Bonkowski, M (2014) Acanthamoeba everywhere: high diversity of Acanthamoeba in soils. Parasitology Research 113, 3151–3158.CrossRefGoogle ScholarPubMed
Graudenz, GS and Dantas, E (2007) Poluição dos ambientes interiores: doenças e sintomas relacionados às edificações. Revista Brasileira de Medicina 2, 23–31.Google Scholar
Hajialilo, E, Behnia, M, Tarighi, F, Niyyati, M and Rezaeian, M (2016) Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran. Parasitology Research 115, 3147–3151.CrossRefGoogle ScholarPubMed
Jercic, MI, Aguayo, C, Saldarriaga-Córdoba, M, Muiño, L, Chenet, SM, Lagos, J, Osuna, A and Fernández, J (2019) Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis. Parasites & vectors 12, 58.CrossRefGoogle ScholarPubMed
Khan, NA, Jarroll, EL and Paget, TA (2002) Molecular and physiological differentiation between pathogenic and non-pathogenic Acanthamoeba. Current Microbiology 45, 197–202.CrossRefGoogle Scholar
Kocazeybek, B (2015) Free living amoebae: Acanthamoeba species pose a great risk for human health. Indian Journal of Medical Microbiology 33, 349–350.Google Scholar
Kumar, S, Stecher, G, Li, M, Knyaz, C and Tamura, K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35, 1547–1549.CrossRefGoogle ScholarPubMed
Ledee, DR, Iovieno, A, Miller, D, Mandal, N, Diaz, M, Fell, J, Fine, ME and Alfonso, EC (2009) Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients. Journal of clinical microbiology 47, 1458–1462.CrossRefGoogle ScholarPubMed
Leung, M and Chan, AHS (2006) Control and management of hospital indoor air quality. Medical Science Monitor 12, 17–23.Google ScholarPubMed
Marciano-Cabral, F, Jamerson, M and Kaneshiro, ES (2010) Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA. Journal of Water and Health 8, 71–82.CrossRefGoogle ScholarPubMed
Maschio, VJ, Corção, G and Rott, MB (2015) Identification of Pseudomonas spp. as amoeba-resistant microorganisms in isolates of Acanthamoeba. Revista do Instituto de Medicina Tropical de São Paulo 57, 81–83.CrossRefGoogle Scholar
Muchesa, P, Leifels, M, Jurzik, L, Hoorzook, KB, Barnard, TG and Bartie, C (2017) Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems. Parasitology Research 116, 155–165.CrossRefGoogle ScholarPubMed
Niyyati, M, Lorenzo-Morales, J, Rahimic, F, Motevalli-Haghia, A, Martín-Navarro, CM, Farnia, S, Valladares, B and Rezaeian, M (2009) Isolation and genotyping of potentially pathogenic Acanthamoeba strains from dust sources in Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene 103, 425–427.CrossRefGoogle ScholarPubMed
Ooi, SS, Mak, JW, Chen, DKF and Ambu, S (2017) The correlation of Acanthamoeba from the ventilation system with other environmental parameters in commercial buildings as possible indicator for indoor air quality. Industrial Health 55, 35–45.CrossRefGoogle ScholarPubMed
Page, FC (1988) A new key to Freshwater and Soil Gymnamoebae. Ambleside, Cumbria, UK: Freshwater Biological Association.Google Scholar
Pussard, M and Pons, R (1977) Morphologies de la paroi kystique et taxonomie du genre Acanthamoeba (Protozoa. Amoebida). Protistologica 13, 557–610.Google Scholar
Rahdar, M, Niyyati, M, Salehi, M, Feghhi, M, Makvandi, M, Pourmehdi, M and Farnia, S (2012) Isolation and genotyping of Acanthamoeba strains from environmental sources in Ahvaz city, Khuzestan province, southern Iran. Iranian Journal of Parasitology 7, 22–26.Google ScholarPubMed
Ross, C, Menezes, JR, Svidzinski, TIE, Albino, U and Andrade, G (2004) Studies on fungal and bacterial population of air-conditioned environments. Brazilian Archives Biology Technology 47, 827–835.CrossRefGoogle Scholar
Santana, WO and Fortuna, JL (2012) Microbiota de aparelhos de ar condicionado das áreas críticas de hospitais públicos e particulares e sua relação com as infecções hospitalares. Revista Biociências 18, 56–64.Google Scholar
Scheikl, U, Tsao, HF and Horn, M (2016) Free-living amoebae and their associated bacteria in Austrian cooling towers: a 1-year routine screening. Parasitology Research 115, 3365.CrossRefGoogle ScholarPubMed
Schroeder, JM, Booton, G, Hay, J, Niszl, IA, Seal, DV, Markus, MB, Fuerst, PA and Byers, TJ (2001) Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoeba from humans with keratitis and from sewage sludge. Journal of Clinical Microbiology 39, 1903–1911.CrossRefGoogle ScholarPubMed
Sente, C, Erume, J, Naigaga, I, Magambo, PK, Ochwo, S, Mulindwa, J, Namara, BG, Kato, CD, Sebyatika, G, Muwonge, K and Ocaido, M (2016) Occurrence and genetic characterisation of Acanthamoeba spp. from environmental and domestic water sources in Queen Elizabeth Protected Area, Uganda. Parasites & Vectors 9, 1–8.CrossRefGoogle Scholar
Siddiqui, R and Khan, NA (2012) Biology and pathogeneses of Acanthamoeba. Parasites and Vectors 5, 6.CrossRefGoogle Scholar
Silva, MA and Rosa, JA (2003) Isolamento de amebas de vida livre potencialmente patogênicas em poeira de hospitais. Revista de Saúde Pública 37, 242–246.CrossRefGoogle Scholar
Silva, DP, Nazaré, DL, Muniz, JWC and Câmara, CNS (2013) Infecções hospitalares associadas à qualidade do ar em ambientes climatizados. Revista de Epidemiologia e Controle de Infecção 3, 153–157.Google Scholar
Todd, CD, Reyes-Batlle, M, Martín-Navarro, CM, Dorta-Gorrín, A, Lopez-Arencibia, A, Martínez-Carretero, E, Piñero, JE, Valladares, B, Lindo, JF and Lorenzo-Morales, J (2015) Isolation and genotyping of Acanthamoeba strains from soil sources from Jamaica, West Indies. Journal of Eukaryotic Microbiology 62, 416–421.CrossRefGoogle ScholarPubMed
Trabelsi, H, Dendana, F, Sellami, A, Sellami, H, Cheikhrouhou, F, Neji, S, Makni, F and Ayadi, A (2012) Pathogenic free-living amoebae: epidemiology and clinical review. Pathologie Biologie 60, 399–405.CrossRefGoogle ScholarPubMed
Trabelsi, H, Dendana, F, Neji, S, Sellami, H, Cheikhrouhou, F, Makni, F and Ayadi, A (2016) Morphological and molecular identification of free living amoeba isolated from hospital water in Tunisia. Parasitology Research 115, 431–435.CrossRefGoogle ScholarPubMed
Visvesvara, GS (2013) Infections with free-living amebae. In Tanowitz, HBG and Brutto, OHD (eds), Handbook of Clinical Neurology. Atlanta, USA: Elsevier, pp. 153–168.Google Scholar
Visvesvara, GS, Moura, H and Schuster, FL (2007) Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunology and Medical Microbiology 50, 1–26.CrossRefGoogle ScholarPubMed
Walochnik, J, Obwaller, A and Aspöck, H (2000) Correlations between morphological, molecular biological and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Applied and Environmental Microbiology 66, 4408–4413.CrossRefGoogle ScholarPubMed
Walochnik, J, Scheikl, U and Haller-Schober, EM (2015) Twenty years of Acanthamoeba diagnostics in Austria. The Journal of eukaryotic microbiology 62, 3–11.CrossRefGoogle ScholarPubMed