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The FMRFamide-like neuropeptide AF2 is present in the parasitic nematode Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

C. D. Keating ,
L. Holden-Dye ,
M. C. Thorndyke ,
R. G. Williams ,
A. Mallett  and
R. J. Walker
C. D. Keating
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
L. Holden-Dye
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
M. C. Thorndyke
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX
R. G. Williams
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
A. Mallett
Affiliation:
St Johns Institute of Dermatology, UMDS St Thomas' Hospital, London SE1 7EH
R. J. Walker
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
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Summary

Peptides belonging to the FMRFamide family are widely distributed amongst invertebrates. We report here on the isolation of the FMRFamide neuropeptide AF2 (Lys-His-Glu-Tyr-Leu-Arg-Phe-NH2) from the parasitic nematode Haemonchus contortus. Immunocytochemical techniques showed that FMRFamide-like material was distributed in several regions of these organisms including nerve cords and cell bodies of the central nervous system. AF2 was isolated using a method that employed 6 steps of reverse-phase HPLC. The concentration of AF2 in this organism was approximately 30 pmol/g of nematode.

Keywords

Haemonchus contortusAF2FMRFamide-related peptidesimmunocytochemistryneuropeptides
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 515 - 521
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Brownlee, D. J. A., Fairweather, I., Johnston, C. F., Smart, D., Shaw, C. & Halton, D. W. (1993 a). Immunocytochemical demonstration of neuropeptides in the CNS of the roundworm, Ascaris suum (Nematoda: Ascaroidea). Parasitology 106, 305–16.CrossRefGoogle Scholar
Brownlee, D. J. A., Fairweather, I., Johnston, C. F. & Shaw, C. (1993 b). Immunocytochemical demonstration of peptidergic and serotoninergic components in the enteric nervous system of the roundworm Ascaris suum (Nematoda, Ascaroidea) Parasitology 106, 305–16.CrossRefGoogle Scholar
Cowden, C., Sithigorngul, P., Brackley, P., Guastella, J. & Stretton, A. O. W. (1993). Localisation and differential expression of FMRFamide-like immunoreactivity in the nematode Ascaris suum. Journal of Comparative Neurology 333, 455–68.CrossRefGoogle ScholarPubMed
Cowden, C. & Stretton, A. O. W. (1993). AF-2, an Ascaris neuropeptide: isolation, sequence and bioactivity. Peptides 14, 423–30.CrossRefGoogle Scholar
Cowden, C., Stretton, A. O. W. & Davis, R. (1989). AF-1, a sequenced bioactive neuropeptide isolated from the nematode Ascaris suum. Neuron 2, 1465–73.CrossRefGoogle Scholar
Davenport, T. R. B., Lee, D. L. & Isaac, R. E. (1988). Immunocytochemical demonstration of a neuropeptide in Ascaris suum (Nematoda) using an antiserum to FMRFamide. Parasitology 97, 81–8.CrossRefGoogle ScholarPubMed
Elphick, M. R., Reeve, J. R. Jr, Burke, R. D. & Thorndyke, M. C. (1991). Isolation of the neuropeptide SLMFamide-1 from starfish using a new antiserum. Peptides 12, 455–9.CrossRefGoogle ScholarPubMed
Franks, C. J., Holden-Dye, L., Williams, R. G., Pang, F. & Walker, R. J. (1994). A nematode FMRFamide-like peptide SDPMFLRFamide (PF-1) relaxes the dorsal muscle strip preparation of Ascaris suum. Parasitology 108, 229–36.CrossRefGoogle Scholar
Geary, T. G., Price, D. A., Bowman, J. W., Winterrowd, C. A., Mackenzie, C. D., Garrison, R. D., Williams, J. F. & Friedman, A. R. (1992). Two FMRFamide-like peptides from the free living nematode Panagrellus redivivus. Peptides 13, 209–14.CrossRefGoogle ScholarPubMed
Holden-Dye, L., Franks, C. J., Williams, R. G. & Walker, R. J. (1995). The effect of the nematode peptides SDPNFLRFamide (PF1) and SADPNFLRFamide (PF2) on synaptic transmission in the parasitic nematode Ascaris suum. Parasitology 110, 449–55.CrossRefGoogle ScholarPubMed
Maule, A. G., Shaw, C., Bowman, J. W., Halton, D. W., Thompson, D. W., Geary, T. G. & Thim, L. (1994 a). The FMRFamide-like neuropeptide AF2 (Ascaris suum) is present in the free-living nematode Panagrellus redivivus (Nematoda, Rhabditida). Parasitology 109, 351–6.CrossRefGoogle ScholarPubMed
Maule, A. G., Shaw, C., Bowman, J. W., Halton, D. W., Thompson, D. W., Geary, T. G. & Thim, L. (1994 b). KSAYMRFamide: A novel FMRFamide-related heptapeptide from the free living nematode, Panagrellus redivivus, which is myoactive in the parasitic nematode Ascaris suum. Biochemical and Biophysical Research Communications 200, 973–80.CrossRefGoogle ScholarPubMed
Maule, A. G., Shaw, C., Bowman, J. W., Halton, D. W., Thompson, D. W., Thim, L., Kubiak, T. M., Martin, R. A. & Geary, T. G. (1995). Isolation and preliminary characterisation of KPNFIRFamide, a novel FMRFamide-related peptide from the free living nematode, Panagrellus redivivus. Peptides 16, 87–93.CrossRefGoogle ScholarPubMed
Moore, S. J. & Thorndyke, M. C. (1993). Immunocytochemical mapping of the novel Echinoderm neuropeptide SALMFamide-1 (SI) in the starfish Asterias rubens. Cell and Tissue Research 274, 605–18.CrossRefGoogle Scholar
Pang, F.-Y., Mason, J., Holden-Dye, L., Franks, C. J., Williams, R. G. & Walker, R. J. (1995). The effects of the nematode peptide, KHEYLRFamide (AF2), on the somatic musculature of the parasitic nematode Ascaris suum. Parasitology 110, 353–62.CrossRefGoogle ScholarPubMed
Price, D. A. (1982). The FMRFamide like peptide of Helix aspersa. Comparative Biochemistry and Physiology 72(C), 325–8.Google Scholar
Rosoff, M. L., Burglin, T. R. & Li, C. (1992). Alternatively spliced transcripts of the flp-1 gene encode distinct FMRFamide-like peptides in Caenorhabditis elegans. Journal of Neuroscience 12, 2356–61.CrossRefGoogle ScholarPubMed
Rosoff, M. L., Doble, K. E., Price, D. A. & Li, C. (1993). The flp-1 propeptide is processed into multiple, highly similar FMRFamide like peptides in Caenorhabditis elegans. Peptides 14, 331–8.CrossRefGoogle ScholarPubMed
Schinkman, K. & Li, C. (1992). Localisation of FMRFamide like peptides in Caenorhabditis elegans. Journal Comparative Neurology 316, 251–60.CrossRefGoogle Scholar
Sithigorngul, P., Stretton, A. O. W. & Cowden, C. (1990). Neuropeptide diversity in Ascaris: an immunocytochemical study. Journal of Comparative Neurology 294, 362–76.CrossRefGoogle ScholarPubMed
Stretton, A. O. W., Cowden, C., Sithigorngul, P. & Davis, R. E. (1991). Neuropeptides in the nematode Ascaris suum. Parasitology 102, S107–S116.CrossRefGoogle ScholarPubMed
Walker, R. J. (1992). Neuroactive peptides with an RFamide or Famide carboxyl terminal. Comparative Biochemistry and Physiology 102C, 213–22.Google ScholarPubMed