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Extraction of microsporidial DNA from modified trichrome-stained clinical slides and subsequent species identification using PCR sequencing

Published online by Cambridge University Press:  28 April 2008

K. S. CHAN  and
T. H. KOH
K. S. CHAN*
Affiliation:
Department of Pathology, Singapore General Hospital, Outram Road, Singapore169608
T. H. KOH
Affiliation:
Department of Pathology, Singapore General Hospital, Outram Road, Singapore169608
*
*Corresponding author. Tel: +65 91804878/+65 63214505. Fax: +65 6222 6826. E-mail address: [email protected]
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Summary

Molecular techniques involving polymerase chain reaction (PCR) and sequencing provide a relatively simple and objective means of identifying microsporidia to species level. We modified previously described methods of DNA extraction and PCR conditions for identification of microsporidia from museum slides, clinical specimens and environmental samples and successfully identified Vittaforma corneae in 11 out of 13 cases of microsporidial infection from used trichrome-stained slides of corneal scrapings from HIV-negative patients with keratoconjunctivitis.

Keywords

microsporidiapolymerase chain reactionDNA extractionkeratoconjunctivitisVittaforma corneae
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 6 , May 2008 , pp. 701 - 703
Copyright
Copyright © 2008 Cambridge University Press

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References

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