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Experimental infection by Haemonchus contortus in lambs: influence of disease on purine levels in serum

Published online by Cambridge University Press:  17 February 2014

LUCAS T. GRESSLER ,
ALEKSANDRO S. DA SILVA ,
CAMILA B. OLIVEIRA ,
ANDRESSA S. SCHAFER ,
ADELINA R. AIRES ,
JOSÉ F. X. ROCHA ,
ALEXANDRE A. TONIN ,
GABRIEL H. SCHIRMBECK ,
EMERSON A. CASALI  and
SONIA T. A. LOPES
...Show all authors
LUCAS T. GRESSLER
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
ALEKSANDRO S. DA SILVA*
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil
CAMILA B. OLIVEIRA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
ANDRESSA S. SCHAFER
Affiliation:
Department of Large Animal, UFSM, Brazil
ADELINA R. AIRES
Affiliation:
Department of Large Animal, UFSM, Brazil
JOSÉ F. X. ROCHA
Affiliation:
Department of Large Animal, UFSM, Brazil
ALEXANDRE A. TONIN
Affiliation:
Department of Small Animal, UFSM, Brazil
GABRIEL H. SCHIRMBECK
Affiliation:
Department of Biochemistry and Department of Morphological Sciences, ICBS, Universidade Federal do Rio Grande do Sul, Brazil
EMERSON A. CASALI
Affiliation:
Department of Biochemistry and Department of Morphological Sciences, ICBS, Universidade Federal do Rio Grande do Sul, Brazil
SONIA T. A. LOPES
Affiliation:
Department of Small Animal, UFSM, Brazil
MARTA L. R. LEAL
Affiliation:
Department of Large Animal, UFSM, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
*
* Corresponding author: Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil. E-mail: [email protected]
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Summary

The aim of this study was to evaluate the purine levels of lambs experimentally infected with Haemonchus contortus. A total of 12 healthy lambs were divided into two groups, composed of 6 animals each: Group A represented the healthy animals (uninfected), while in Group B the animals were infected with 15 000 larvae of H. contortus. Blood was drawn on days 15, 45 and 75 post-infection (PI) in order to perform the purine analysis (ATP, ADP, AMP, adenosine, inosine, hypoxanthine, xanthine and uric acid) by high pressure liquid chromatography (HPLC) in serum. On day 15 PI a significant (P<0·05) increase in the levels of ATP and inosine was observed in the infected animals, unlike the levels of ADP, adenosine, xanthine and uric acid which were reduced. On day 45 PI a significant (P<0·05) increase in the ATP and xanthine levels in infected animals was observed, contrasting with reduced levels of ADP and uric acid. Finally, on day 75 PI an increase occurred in the levels of ATP, adenosine and hypoxanthine in infected lambs, concomitant with a reduction in the levels of ADP and uric acid (P<0·05). These changes in purine levels may influence the inflammatory process and the pathological events.

Keywords

SheepTrichostrongylidaepurinergic systemATPADPadenosineuric acid
Type
Research Article
Information
Parasitology , Volume 141 , Issue 7 , June 2014 , pp. 898 - 903
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Copyright
Copyright © Cambridge University Press 2014 

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