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The excystation of Entamoeba histolytica without bacteria in microcultures

Published online by Cambridge University Press:  07 April 2009

Charles W. Rees ,
Lucy V. Reardon  and
Ida Louise Bartgis
Charles W. Rees
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
Lucy V. Reardon
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
Ida Louise Bartgis
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
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Extract

1. Formulae developed by Anfinsen et al. (1946) for a medium used in the cultivation of Plasmodium knowlesi were used for media in which excystation of Entamoeba histolytica without bacteria was investigated.

2. The following media were used: (i) an inorganic fluid containing carbon dioxide and chlorides, phosphates, and bicarbonates, of sodium, potassium, magnesium, and calcium; (ii) an inorganic fluid of chlorides and phosphates of the above metals without bicarbonates; (iii) the same respective fluids plus glucose, and (iv) the same plus enrichments with B vitamins, cocarboxylase, vitamin C, purines, pyrimidines, folic acid, glucosamine, cholesterol, and amino-acids. The oxygen content of all media was lowered either by cysteine or glutathione.

3. Some excystation occurred in all media, the percentages were lowest in the inorganic fluid without bicarbonates, highest in fluid with bicarbonates plus all of the listed organic compounds, and intermediate in the inorganic bicarbonate fluid plus glucose. Amino-acids were not required for good percentages of excystation.

4. Excystation occurred when cysts were isolated in medium with Trypanosoma cruzi.

5. The data show that organic compounds in the medium are necessary for high percentages of excystation.

Type
Research Article
Information
Parasitology , Volume 40 , Issue 3-4 , October 1950 , pp. 338 - 342
Copyright
Copyright © Cambridge University Press 1950

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References

Anfinsen, C. B., Geiman, Q. M., McKee, R. W., Obmsbee, B. A. & Ball, E. G. (1946). Studies on malarial parasites. VIII. Factors affecting the growth of Plasmodium, knowlesi in vitro. J. Exp. Med. 84, 607.Google Scholar
Bayliss, W. M. (1924). Principles of General Physiology. London: Longmans, Green and Co.Google Scholar
Cleveland, L. R. & Sanders, E. P. (1930 a). The production of bacteria-free amoebic abscesses in the liver of eats and observations on the amoebae in various media with and without bacteria. Science, 72, 149.Google Scholar
Cleveland, L. R. & Sanders, E. P. (1930 b). Encystation, multiple fission without encystment, excystation, metacystic development, and variation in a pure line and nine strains of Entamoeba histolytica. Arch. Protistenk. 70, 223.Google Scholar
Dobell, C. (1927). Further observations and experiments on the cultivation of Entamoeba histolytica from cysts. Parasitology, 19, 288.Google Scholar
Dobell, C. (1928). Researches on the intestinal protozoa of monkeys and man. I. General introduction. II. Description of the whole life-history of Entamoeba histolytica in cultures. Parasitology, 20, 357.CrossRefGoogle Scholar
Dobell, C. (1931). IV. An experimental study of the histolytica-like species of Entamoeba living naturally in macaques. Parasitology, 23, 1.Google Scholar
Dobell, C. & Laidlaw, P. P. (1926). On the cultivation of Entamoeba histolytica and some other entozoic amoebae. Parasitology, 18, 283.Google Scholar
Eulek, H., von Nilsson, R. & Auhagen, E. (1931). Ueber die Function des Magnesiums beim enzymatischen Kohlenhydratbau. Hoppe-Seyl. Z. 200, 2.Google Scholar
Faust, E. C. (1940). The carrier races of Endamoeba histolytica in a New Orleans Children's Institution. J. Parasit. Supp. 26, 21.Google Scholar
Meleney, H. E., Frye, W. W., Leathers, W. S. & Snyder, T. L. (1940). The sterilization of the cysts of Endamoeba histolytica, with preliminary observations on subsequent excystation. Proc. 3rd Int. Congr. Microbiol, p. 410.Google Scholar
Phillips, B. P. (1950). The cultivation of Endamoeba histolytica with Trypanosoma cruzi. Science, 111, 8.CrossRefGoogle ScholarPubMed
Porter, J. R. (1947). Bacterial Chemistry and Physiology. London: John Wiley and Sons.CrossRefGoogle Scholar
Quastel, J. H. & Webley, D. M. (1942). Vitamin B1 and bacterial oxidations. 2. The effects of magnesium, potassium, and hexose diphosphate ions. Biochem. J. 36, 8.CrossRefGoogle Scholar
Rees, C. W. (1942). The construction of a micromanipulator for the isolation of protozoa. Amer. J. Trop. Med. 22, 487.Google Scholar
Rees, C. W., Reardon, H. D. & Baeknstein, L. V. (1948). A biochemical approach to the problem of cultivating Endamoeba histolytica without bacteria. J. Parasitol. 34 (Sec. 2), 11.Google Scholar
Rees, C. W. & Reardon, L. V., Jones, F. E., Griffin, A. M. (1947). Observations on the excystation of Endamoeba histolytica. J. Parasit. 33, 385.Google Scholar
Snyder, T. L. & Meleney, H. E. (1941). The excystation of Endamoeba histolytica in bacteriologically sterile media. Amer. J. Trop. Med. 21, 63.CrossRefGoogle Scholar
Stephenson, M. (1949). Bacterial Metabolism, 3rd ed.London: Longmans, Green and Co.Google Scholar
Sttmner, J. B. & Somers, G. F. (1947). Chemistry and Methods of Enzymes. New York: Academic Press Inc.Google Scholar
Yorke, W. & Adams, A. R. D. (1926). Observations on Entamoeba histolytica. I. Development of cysts, excystation, and development of excysted amoebae in vitro. Ann. Trop. Med. Parasit. 20, 279.Google Scholar