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Eukaryotic expression of recombinant Pso o 1, an allergen from Psoroptes ovis, and its localization in the mite

Published online by Cambridge University Press:  18 September 2006

A. J. NISBET ,
A. MacKELLAR ,
K. McLEAN ,
G. P. BRENNAN  and
J. F. HUNTLEY
A. J. NISBET
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland
A. MacKELLAR
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland
K. McLEAN
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland
G. P. BRENNAN
Affiliation:
School of Biology and Biochemistry, Queen's University Belfast, Lisburn Road, Belfast BT9 7BL, Northern Ireland
J. F. HUNTLEY
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland
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Abstract

A cDNA encoding the immunogen Pso o 1 from Psoroptes ovis was obtained by polymerase chain reaction (PCR) amplification. The amplicon contained the entire coding sequence for the prepro-enzyme in an open reading frame (ORF) of 966 bp. This gene encoded a predicted protein of 322 amino acids (aa) with 64% aa identity (80% similarity) to the major house dust mite faecal allergen Der f 1. The pro-enzyme form of Pso o 1 was expressed as a recombinant protein in the Pichia pastoris-eukaryotic expression system. Maturation of the recombinant pro-enzyme by autocatalytic activation was not observed, and such maturation could not be achieved using a number of techniques known to activate recombinant Der p 1 and Der f 1 expressed in the same system. Serum raised against recombinant Pso o 1 cross-reacted with mature Der p 1 and allowed Pso o 1 to be immunolocalized to the gut of P. ovis.

Keywords

PsoroptesallergenPso o 1cysteine proteinase
Type
Research Article
Information
Parasitology , Volume 134 , Issue 1 , January 2007 , pp. 83 - 89
Copyright
© 2006 Cambridge University Press

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