Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-12-05T02:14:46.410Z Has data issue: false hasContentIssue false
  • English
  • Français

Entamoeba chiangraiensis n. sp. (Amoebozoa: Entamoebidae) isolated from the gut of Asian swamp eel (Monopterus albus) in northern Thailand

Published online by Cambridge University Press:  23 October 2019

Vasana Jinatham ,
Siam Popluechai ,
C. Graham Clark  and
Eleni Gentekaki Open the ORCID record for Eleni Gentekaki [Opens in a new window]
Vasana Jinatham
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand
Siam Popluechai
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, 57100Thailand
C. Graham Clark
Affiliation:
Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Eleni Gentekaki*
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, 57100Thailand
*
Author for correspondence: Eleni Gentekaki, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The genus Entamoeba comprises mostly gut parasites and commensals of invertebrate and vertebrate animals including humans. Herein, we report a new species of Entamoeba isolated from the gut of Asian swamp eels (Monopterus albus) in northern Thailand. Morphologically, the trophozoite is elongated and has a single prominent pseudopodium with no clear uroid. The trophozoite is actively motile, 30–50 µm in length and 9–13 µm in width. Observed cysts were uninucleate, ranging in size from 10 to 17.5 µm in diameter. Chromatin forms a fine, even lining along the inner nuclear membrane. Fine radial spokes join the karyosome to peripheral chromatin. Size, host and nucleus morphology set our organism apart from other members of the genus reported from fish. The SSU rRNA gene sequences of the new isolates are the first molecular data of an Entamoeba species from fish. Phylogenetic analysis places the new organism as sister to Entamoeba invadens. Based on the distinct morphology and SSU rRNA gene sequence we describe it as a new species, Entamoeba chiangraiensis.

Keywords

Archamoebaeintestinal protistmorphologyphylogenySSU rRNA
Type
Research Article
Information
Parasitology , Volume 146 , Special Issue 14: BSP 2018 Autumn Symposium , December 2019 , pp. 1719 - 1724
Check for updates
Copyright
Copyright © Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

ZooBank ID: urn:lsid:zoobank.org:act:1BAAFBDF-137F-4046-A196-76C2B5D1D70A

References

Alexeieff, A (1912) Sur les charactères cytologiques et la systématique des amibes du groupe Limax (Naegleria nov. gen. et Hartmannia nov. gen.) et des amibes parasites des Vertèbres (Proctamoeba nov. gen). Bulletin de la Société Zoologique de France 37, 55–74.Google Scholar
Bullock, WL (1966) Entamoeba gadi sp. n. from the rectum of the pollock, Pollachius virens (L., 1758), with some observations on its cytochemistry. The Journal of Parasitology 52, 679–684.Google Scholar
Capella-Gutierrez, S, Silla-Martinez, JM and Gabaldon, T (2009) Trimal: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics (Oxford, England) 25, 1972–1973.Google Scholar
Casagrandi, O and Barbagallo, P (1895) Ricerche biologiche e cliniche suli’ Amoeba coli (Lösch) (Nota preliminare). Bullettino delle Sedute della Accademia Gioenia di Scienze Naturali in Catania 39, 4.Google Scholar
Chen, C (1955) The protozoan parasites from four species of Chinese pond fishes: Ctenopharyngodon idellus, Mylopharyngodon aethiops, Aristichthys nobilis and Hypophthalmichthys molitrix. I. The protozoan parasites of Ctenopharyngodon idellus. Acta Hydrobiologica Sinica 2, 123–164.Google Scholar
Clark, CG and Diamond, LS (1997) Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting. Journal of Eukaryotic Microbiology 44, 142–154.Google Scholar
Clark, CG and Stensvold, CR (2015) The continuously expanding universe of Entamoeba. In Nozaki, T, Bhattacharya, A (eds), Amebiasis. Tokyo: Springer, pp. 9–25.Google Scholar
Clark, CG, Kaffashian, F, Tawari, B, Windsor, JJ, Twigg-Flesner, A, Davies-Morel, MC, Blessmann, J, Ebert, F, Peschel, B, Le Van, A, Jackson, CJ, Macfarlane, L and Tannich, E (2006) New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes. International Journal of Systematic and Evolutionary Microbiology 56, 2235–2239.Google Scholar
da Cunha, A and Penido, JCN (1926) Entamoeba pimelodi, n. sp., parasite d'un poisson d'eau douce. Comptes Rendus des Seances de la Société de Biologie 95, 1010–1011.Google Scholar
Diamond, LS (1982) A new liquid medium for xenic cultivation of Entamoeba histolytica and other lumen-dwelling protozoa. The Journal of Parasitology 68, 958–959.Google Scholar
Fotedar, R, Stark, D, Beebe, N, Marriott, D, Ellis, J and Harkness, J (2007 a) Laboratory diagnostic techniques for Entamoeba species. Clinical Microbiology Reviews 20, 511–532.Google Scholar
Fotedar, R, Stark, D, Beebe, N, Marriott, D, Ellis, J and Harkness, J (2007 b) PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia. Journal of Clinical Microbiology 45, 1035–1037.Google Scholar
García, G, Ramos, F, Pérez, RG, Yañez, J, Estrada, MS, Mendoza, LH, Martinez-Hernandez, F and Gaytán, P (2014) Molecular epidemiology and genetic diversity of Entamoeba species in a chelonian collection. Journal of Medical Microbiology 63, 271–283.Google Scholar
Ghabanchi, J, Zibaei, M, Afkar, MD and Sarbazie, AH (2010) Prevalence of oral Entamoeba gingivalis and Trichomonas tenax in patients with periodontal disease and healthy population in Shiraz, southern Iran. Indian Journal of Dental Research 21, 89–91.Google Scholar
Ghosh, TN (1973) Studies on the Genus Entamoeba: I. E. paulista (carini) Stabler & Chen, 1936. Archiv für Protistenkunde 115, 162–167.Google Scholar
Gonin, P and Trudel, L (2003) Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 41, 237–241.Google Scholar
Hooshyar, H, Rostamkhani, P and Rezaeian, M (2015) An annotated checklist of the human and animal Entamoeba (amoebida: Endamoebidae) species- A review article. Iranian Journal of Parasitology 10, 146–156.Google Scholar
Imam, EA, Ramadan, EI and Derahilli, FS (1987) On some internal protozoa infecting some Nile fishes in Egypt. Journal of the Egyptian Veterinary Medical Association 47, 55–61.Google Scholar
Jacob, AS, Busby, EJ, Levy, AD, Komm, N and Clark, CG (2015) Expanding the Entamoeba universe: new hosts yield novel ribosomal lineages. Journal of Eukaryotic Microbiology 63, 69–78.Google Scholar
Katoh, K and Toh, H (2010) Parallelization of the MAFFT multiple sequence alignment program. Bioinformatics (Oxford, England) 26, 1899–1900.Google Scholar
Kawano, T, Imada, M, Chamavit, P, Kobayashi, S, Hashimoto, T and Nozaki, T (2017) Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. PLoS One 12, e0185233.Google Scholar
Loftus, B, Anderson, I, Davies, R, Alsmark, UC, Samuelson, J, Amedeo, P, Roncaglia, P, Berriman, M, Hirt, RP, Mann, BJ, Nozaki, T, Suh, B, Pop, M, Duchene, M, Ackers, J, Tannich, E, Leippe, M, Hofer, M, Bruchhaus, I, Willhoeft, U, Bhattacharya, A, Chillingworth, T, Churcher, C, Hance, Z, Harris, B, Harris, D, Jagels, K, Moule, S, Mungall, K, Ormond, D, Squares, R, Whitehead, S, Quail, MA, Rabbinowitsch, E, Norbertczak, H, Price, C, Wang, Z, Guillén, N, Gilchrist, C, Stroup, SE, Bhattacharya, S, Lohia, A, Foster, PG, Sicheritz-Ponten, T, Weber, C, Singh, U, Mukherjee, C, El-Sayed, NM, Petri, WA Jr, Clark, CG, Embley, TM, Barrell, B, Fraser, CM and Hall, N (2005) The genome of the protist parasite Entamoeba histolytica. Nature 433, 865–868.Google Scholar
Luszczak, J, Bartosik, M, Rzymowska, J, Sochaczewska-Dolecka, A, Tomaszek, E, Wysokinska-Miszczuk, J and Bogucka-Kocka, A (2016) The occurrence of Entamoeba gingivalis among patients with periodontal disease. Current Issues in Pharmacy and Medical Sciences 29, 86–89.Google Scholar
Maybodi, FR, Ardakani, AH, Bafghi, AF, Ardakani, AH and Zafarbakhsh, A (2016) The effect of nonsurgical periodontal therapy on Trichomonas tenax and Entamoeba gingivalis in patients with chronic periodontitis. Journal of Dentistry 17, 171–176.Google Scholar
Noble, ER and Noble, GA (1966) Amebic parasites of fishes. The Journal of Protozoology 13, 478–480.Google Scholar
Orias, JD and Noble, ER (1971) Entamoeba nezumia sp. n. and other parasites from a North Atlantic fish. The Journal of Parasitology 57, 945–947.Google Scholar
Pánek, T, Zadrobílková, E, Walker, G, Brown, MW, Gentekaki, E, Hroudová, M, Kang, S, Roger, AJ, Tice, AK, Vlček, Č and Čepička, I (2016) First multigene analysis of Archamoebae (Amoebozoa: Conosa) robustly reveals its phylogeny and shows that Entamoebidae represents a deep lineage of the group. Molecular Phylogenetics and Evolution 98, 41–51.Google Scholar
Ptáčková, E, Kostygov, AY, Chistyakova, LV, Falteisek, L, Frolov, AO, Patterson, DJ, Walker, G and Cepicka, I (2013) Evolution of Archamoebae: morphological and molecular evidence for pelobionts including Rhizomastix, Entamoeba, Iodamoeba, and Endolimax. Protist 164, 380–410.Google Scholar
Santos, HL, Bandea, R, Martins, LA, de Macedo, HW, Peralta, RH, Peralta, JM, Ndubuisi, MI and da Silva, AJ (2010) Differential identification of Entamoeba spp. based on the analysis of 18S rRNA. Parasitology Research 106, 883–888.Google Scholar
Shilton, CM, Šlapeta, J, Shine, R and Brown, GP (2018) Invasive colonic entamoebiasis in wild Cane Toads, Australia. Emerging Infectious Diseases 24, 1541–1543.Google Scholar
Shiratori, T and Ishida, KI (2015) Entamoeba marina n. sp.; a new species of Entamoeba isolated from tidal flat sediment of Iriomote Island, Okinawa, Japan. Journal of Eukaryotic Microbiology 63, 280–286.Google Scholar
Silberman, JD, Clark, CG, Diamond, LS and Sogin, ML (1999) Phylogeny of the genera Entamoeba and Endolimax as deduced from small-subunit ribosomal RNA sequences. Molecular Biology and Evolution 16, 1740–1751.Google Scholar
Stamatakis, A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics (Oxford, England) 22, 2688–2690.Google Scholar
Stensvold, CR, Lebbad, M, Victory, EL, Verweij, JJ, Tannich, E, Alfellani, M, Legarraga, P and Clark, CG (2011) Increased sampling reveals novel lineages of Entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection. Protist 162, 525–541.Google Scholar
Taliaferro, WH and Holmes, F (1924) Entamoeba barreti, n. sp., from the turtle Chelydra serpentina; a description of the amoeba from the vertebrate host and from Barret and Smith's cultures. American Journal of Epidemiology 4, 160–168.Google Scholar
Verweij, JJ, Polderman, AM and Clark, CG (2001) Genetic variation among human isolates of uninucleated cyst-producing Entamoeba species. Journal of Clinical Microbiology 39, 1644–1646.Google Scholar
Verweij, JJ, Laeijendecker, D, Brienen, EA, van Lieshout, L and Polderman, AM (2003) Detection and identification of Entamoeba species in stool samples by a reverse line hybridization assay. Journal of Clinical Microbiology 41, 5041–5045.Google Scholar
Supplementary material: PDF

Jinatham et al. supplementary material

Jinatham et al. supplementary material

Download Jinatham et al. supplementary material(PDF)
PDF 45.5 KB