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Electrophoretic studies on enzymes of diploid and triploid paragonimus westermani

Published online by Cambridge University Press:  06 April 2009

Takeshi Agatsuma  and
Shigehisa Habe
Takeshi Agatsuma
Affiliation:
Department of Parasitology, Kochi Medical School, Oko-cho Nankoku 781–51, Japan
Shigehisa Habe
Affiliation:
Department of Parasitology, School of Medicine, Fukuoka University, Fukuoka 814–01, Japan
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Summary

An enzyme analysis of diploid and triploid Paragonimus westermani was conducted using starch gel electrophoresis. In total, 16 enzymes, probably encoded by 18 loci, were studied for 3 populations of the diploid form sampled from 2 localities, and 4 populations of the triploid form from 4 localities. Comparison of the enzymes of the triploid and the diploid digeneans showed 5 different patterns; diaphorase (EC 1.6.2.2), glutamic-oxaloacetic transaminase (EC 2.6.1.1), hexokinase (EC 2.7.1.1), leucylglycylglycine aminopeptidase (EC 3.4.1.3), and phosphoglucomutase (EC 2.7.5.1). On the basis of the numbers of bands and their patterns, all individuals of the triploid are probably heterozygous at each of these 5 loci and homozygous at the remaining 13 loci. The occurrence of fixed heterozygotes found in triploid populations cannot be easily explained by only a single mutation. It is suggested that the variability may have been introduced by hybridization with a different sub-species or a closely related species and may, thus, have been maintained since the time of the origin of triploids.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 3 , December 1985 , pp. 489 - 497
Copyright
Copyright © Cambridge University Press 1985

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