Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-04T20:18:55.676Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of mebendazole on the encysted phase of Trichinella spiralis in the rat: an electron-microscope study

Published online by Cambridge University Press:  06 April 2009

Sonja De Nollin ,
M. Borgers ,
O. Vanparijs  and
H. Van Den Bossche
Sonja De Nollin
Affiliation:
Janssen Pharmaceutica, Research Laboratories, B 2340 Beerse, Belgium
M. Borgers
Affiliation:
Janssen Pharmaceutica, Research Laboratories, B 2340 Beerse, Belgium
O. Vanparijs
Affiliation:
Janssen Pharmaceutica, Research Laboratories, B 2340 Beerse, Belgium
H. Van Den Bossche
Affiliation:
Janssen Pharmaceutica, Research Laboratories, B 2340 Beerse, Belgium
Get access Rights & Permissions [Opens in a new window]

Extract

The ultrastructural changes in the encysted phase of Trichinella larvae, during and after treatment of the rat with mebendazole, were studied.

The earliest modifications in the infected area concerned the immediate larval environment (matrix). These degenerative changes in the matrix were seen before any alteration could be observed in the larvae.

Subsequently, oedematous swelling, intracellular vacuolization and loss of glycogen were noted in the larval cells.

Together with the progressive involution of the larvae and their matrix, inflammatory and immunocompetent cells invade the infected area and penetrate the altered capsule. Calcification of the larvae and massive infiltration of mononuclear phagocytes with subsequent elimination of the calcified remnants were the prominent features in the first weeks after treatment. The infected areas were completely cleared from larval and matrix residues 50 days after the end of treatment and a nearly normalized tissue was seen from day 111 after treatment.

Type
Research Article
Information
Parasitology , Volume 69 , Issue 1 , August 1974 , pp. 55 - 62
Copyright
Copyright © Cambridge University Press 1974

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beckett, E. B. & Boothroyd, B. (1961). Some observations on the fine structure of the mature larvae of the nematode Trichinella spiralis. Annals of Tropical Medicine and Parasitology 55, 116–24.CrossRefGoogle ScholarPubMed
Bruce, R. (1970). The structure and composition of the capsule of Trichinella spiralis in host muscle. Parasitology 60, 223–7.CrossRefGoogle ScholarPubMed
Brugmans, J. P., Thienpont, D. C., Van Wijngaarden, I., Vanparijs, O. F., Schuermans, V. L. & Lauwers, H. L. (1971). Radiochemical and pilot clinical study in 1278 subjects. Journal of the American Medical Association 217, 313–6.CrossRefGoogle Scholar
Chaia, G. & Da Cunha, A. S. (1971). Therapeutic action of mebendazole against human helminthiasis. Fôlha Medica 63, 843–52.Google Scholar
De Nollin, S. & Van den Bossche, H. (1973). Biochemical effects of mebendazole on Trichinella spiralis larvae. Journal of Parasitology 59, 970–6.CrossRefGoogle ScholarPubMed
Fasske, K. & Themann, H. (1961). Elektronenmikroskopische Befunde an der Muskelfaser nach Trichinenbefall. Virchows Archiv A für Pathologische Anatomie 334, 459–74.CrossRefGoogle Scholar
Garbuliński, T., Kozar, Z., Bubień, Z., Bebowy, J. & Bieliński, E. (1965). P32 incorporation into energetic phosphatases of the heart and skeletal muscles in rats infected with T. spiralis. Acta Parasitologica Polonica 13, 275–83.Google Scholar
Gatti, F., Krubwa, F., Lontie, M., Vandepitte, J. & Thienpont, D. (1971). Congress on Tropical Pathology, Prague, 26 August, 1971.Google Scholar
Kozar, Z., Zarzycki, J. & Kozar, M. (1966). Morphologic observations of Trichinella infected muscles in mice treated with Thiabendazole and Neguvon. Wiadomosci Parazytologiczne 12, 589–604.Google ScholarPubMed
Kozar, Z., Zarzycki, J., Senivta, R. & Martynowicz, T. (1967). Histochemical study of drug effects on mice infected with T. spiralis. Experimental Parasitology 21, 173–85.CrossRefGoogle Scholar
Ribas-Mujal, D. & Rivera-Pomar, J. M. (1968). Biological significance of the early structural alterations in skeletal muscle fibers infected by Trichinella spiralis. Virchows Archiv A für Pathologische Anatomie 345, 154–68.CrossRefGoogle ScholarPubMed
Teppema, J. S., Robinson, J. E. & Ruttenberg, E. J. (1973). Ultrastructural aspects of capsule formation in Trichinella spiralis infection in the rat. Parasitology 66, 291–6.CrossRefGoogle ScholarPubMed
Thienpont, D., Vanparijs, O. F. & Vandesteene, R. (1973). Anthelmintic and histopathological effects of mebendazole on Trichinella spiralis in the rat. Experimental Parasitology. (In the Press.)Google Scholar
Van den Bossche, H. (1972). Biochemical effects of the anthelmintic drug mebendazole. In Comparative Biochemistry of Parasites (ed. H., Van den Bossche), pp. 139–57. New York: Academic Press.CrossRefGoogle Scholar
Van den Bossche, H. & De Nollin, S. (1973). Effects of Mebendazole on the absorption of low molecular weight nutrients by Ascaris suum. International Journal of Parasitology 3, 401–7.CrossRefGoogle ScholarPubMed
Vandepitte, J., Gatti, F., Lontie, M., Krubwa, F., Nguete, M. & Thienpont, D. (1973). Le mebendazole, un nouvel anthelmintique à large spectre très actif contre le trichocephale. Bulletin de la Société de Pathologie exotique 66, 165–78.Google Scholar
Van Reempts, J. & Borgers, M. (1974). A simple polychrome stain for conventionally fixed, Epon-embedded tissues. (Submitted.)Google Scholar