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Effects of infection with Echinostoma paraensei on the circulating haemocyte population of the host snail Biomphalaria glabrata

Published online by Cambridge University Press:  06 April 2009

S. Noda  and
E. S. Loker
S. Noda
Affiliation:
Department of Biology, The University of New Mexico, Albuquerque, New Mexico 87131, USA
E. S. Loker*
Affiliation:
Department of Biology, The University of New Mexico, Albuquerque, New Mexico 87131, USA
*
†Reprint requests to Dr E. S. Loker.
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Summary

Circulating haemocytes from Echinostoma paraensei-infected M line Biomphalaria glabrata snails, or from age- and sizematched control snails, were studied on plastic slides with phase-contrast optics. Granulocytes, hyalinocytes, and round cells were consistently present; granulocytes were further categorized as ‘fully spread' (FS) or ‘partially spread' (PS). Among control snails, the relative percentage and estimated number/mm3 of round cells declined significantly with increased snail size, and the corresponding values for both categories of granulocytes increased. At 1 day post-infection (p.i.) with E. paraensei, overall composition of the haemocyte population was relatively unaffected, but by 8 days p.i. infected snails had significantly higher relative percentages of round cells and PS granulocytes than controls. Because a marked increase in the number of circulating haemocytes is also evident by 8 days p.i., infected snails had approximately 12 times more round cells and PS granulocytes/mm3 of haemolymph than did controls. At 30 days p.i. the relative and absolute abundance of PS granulocytes was still significantly elevated, but otherwise haemocyte populations did not differ from control snails. Alterations in granulocyte size in infected snails were also noted. Infection with E. paraensei has a striking impact of circulating haemocyte populations and also increases the relative concentration of haemocytes with less ability to adhere to a foreign surface.

Type
Research Article
Information
Parasitology , Volume 98 , Issue 1 , February 1989 , pp. 35 - 41
Copyright
Copyright © Cambridge University Press 1989

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