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The effects of gamma-irradiation on migration and survival of Schistosoma mansoni schistosomula in mice

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle ,
T. Dobinson  and
E. R. James
Q. D. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
T. Dobinson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
E. R. James
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
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Summary

Infections with irradiated Schistosoma mansoni were established by intramuscular (i.m.) injection of mechanically transformed schistosomula. A dose of 2·3 krad. allowed persistence of a small proportion of worms to adulthood, and of these survivors the majority of the female worms were sexually sterile. However, a small proportion of 2·3 krad. irradiated females and a larger proportion of similarly irradiated males were capable of pairing successfully with non-irradiated partners. Radiation in the range 2·3 to 10 krad. resulted in slightly reduced peak recoveries from the lungs while 20 krad. resulted in a much reduced and 40 krad. a virtual absence of survival to the lung stage. Increasing doses of radiation in the range 2·3 to 10 krad. resulted in successively fewer parasites reaching the liver. Thus, the major sites of the radiation-induced mortality appeared to be as follows: 2·3 krad., mainly in the liver; 4 krad., in the lungs and liver; 10 krad., mainly in the lungs; 20 krad., at the injection site and in the lungs and 40 krad., mainly at the injection site. The infections studied here showed reduced survival following exposure to high doses of radiation compared with the infections, established as percutaneously applied cercariae, which have been reported by other workers. Possible reasons for the disparity are discussed.

Type
Research Article
Information
Parasitology , Volume 79 , Issue 2 , October 1979 , pp. 223 - 230
Copyright
Copyright © Cambridge University Press 1979

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References

REFERENCES

Bickle, Q. D., Taylor, M. G., Doenhoff, M. J. & Nelson, G. S. (1979). Immunization of mice with gamma-irradiated, intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.Google Scholar
Clegg, J. A. (1965). In vitro cultivation of Schistosoma mansoni. Experimental Parasitology 16, 133–47.Google Scholar
Doenhoff, M., Bickle, Q., Long, B., Bain, J. & McGregor, A. (1978). Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. I. Demonstration of resistance to reinfection using a model system that involves perfusion of mice within three weeks of challenge. Journal of Helminthology 52, 173–86.Google Scholar
Erickson, D. G. (1965). The fate of gamma-irradiated Schistosoma mansoni cercariae in mice. American Journal of Tropical Medicine and Hygiene 14, 574–8.CrossRefGoogle Scholar
James, E. R. & Taylor, M. G. (1976). Transformation of cercariae to schistosomula: a quantitative comparison of the transformation techniques and of infectivity by different injection routes of the organisms produced. Journal of Helminthology 50, 223–33.Google Scholar
Lewis, F. A. & Colley, D. G. (1977). Modification of the lung recovery assay for schistosomula and correlations with worm burdens in mice infected with Schistosoma mansoni. Journal of Parasitology 63, 413–18.Google Scholar
Miller, P. (1976). Migration of the human blood fluke Schistosoma mansoni, and its subsequent development in the mammalian host. D.Phil. thesis, University of York.Google Scholar
Minard, P., Dean, D. A., Jacobson, R. H., Vannier, W. E. & Murrell, K. D. (1978 a). Immunization of mice with Cobalt-60 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.Google Scholar
Minard, P., Dean, D. A., Vannier, W. E. & Murrell, K. D. (1978 b). Effects of immunization on migration of Schistosoma mansoni through lungs. American Journal of Tropical Medicine and Hygiene 27, 87–93.Google Scholar
Mulligan, W. (1975). The preparation of radiation-attenuated vaccines against hehminthic infections. In Nuclear Techniques in Helminthology Research, Panel Proceedings, Nairobi (1973), pp. 1–12. Vienna: International Atomic Energy Agency.Google Scholar
Perlowagora-Szumlewicz, A. (1964). O papel das cercárias atenuades na imunização efetiva contra o Schistosoma mansoni (Studies on acquired resistance to Schistosoma mansoni in mice exposed to x-irradiated cercariae). Revista Brazileira de Malariologia e Doenças Tropicas (Rio de Janeiro) 16, 502–25. (English summary.)Google Scholar
Radke, M. G. & Sadun, E. H. (1963). Resistance produced in mice by exposure to irradiated Schistosoma mansoni cercariae. Experimental Parasitology 13, 134–43.Google Scholar
Smithers, S. R. (1962). Immunizing effect of irradiated cercariae of Schistosoma mansoni in rhesus monkeys. Nature, London 194, 1146–7.Google Scholar
Villella, J. B., Gomberg, H. J. & Gould, S. E. (1961). Immunization to Schistosoma mansoni in mice inoculated with radiated cercariae. Science 134, 1073–5.Google Scholar
Villella, J. B. & Weinbren, M. P. (1965). Abnormalities in adult Schistosoma mansoni developed from gamma irradiated cercariae. Journal of Parasitology 51, 42 (Abstract).Google Scholar
Webbe, G. & James, C. (1972). Host–parasite relationships of Bulinus globosus and B. truncatus with strains of Schistosoma haematobium. Journal of Helminthology 46, 185–99.Google Scholar