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The effect of some metabolic inhibitors on the oxygen uptake of Trypanosoma lewisi and Trypanosoma equiperdum

Published online by Cambridge University Press:  06 April 2009

June P. Thurston
June P. Thurston
Affiliation:
Molteno Institute, University of Cambridge*
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1. The oxygen uptake of Trypanosoma lewisi and T. equiperdum was measured with differential manometers using microflasks of 5 ml. capacity. Each flask contained 2–5 × 107 trypanosomes.

2. The oxygen uptake of trypanosomes in heparinized infected blood was compared with that of washed trypanosomes suspended in Ringer–phosphate–glucose solution with or without added serum or blood. With T. lewisi, the oxygen uptake began at a higher rate in the presence of blood or serum than with Ringer–phosphate–glucose solution. With T. equiperdum, the oxygen uptake in the presence of blood or serum began at almost the same rate as with Ringer–phosphate–glucose solution, but the rate of oxygen uptake decreased more rapidly in the absence of blood or serum than when it was present.

3. High concentrations of iodoacetic acid (e.g. 3·3 × 10−2m) caused an increase in oxygen uptake when tipped into heparinized normal rat blood. Similar concentrations caused an inhibition of oxygen uptake when 2·0 × 10−4m methylene blue was also present or when the blood had been treated with sodium nitrite to convert haemoglobin into methaemoglobin.

4. Iodoacetic acid caused little inhibition of oxygen uptake when T. lewisi was suspended in Ringer–phosphate solution containing glutamine or glutamine plus glucose, or when heparinized infected blood was tested. Inhibition was greatest when the substrate was glucose, while glucosamine, glycerol, or glucose plus serum gave intermediate results.

5. Iodoacetic acid caused least inhibition of oxygen uptake when T. equiperdum was suspended in Ringer–phosphate solution containing glycerol, or when heparinized infected blood was tested. Inhibition was greatest when the substrate was glucose, and was slightly less marked when the substrate was glucose plus serum or glucose plus glycerol.

6. Neoarsphenamine inhibited the oxygen uptake of T. lewisi to a similar extent whether glucose or glutamine was present.

7. Neoarsphenamine and reduced stovarsol thioglycollate inhibited the oxygen uptake of T. equiperdum slightly less when the substrate was glycerol than when it was glucose. Sodium arsenite was as active as reduced stovarsol thioglycollate when the substrate was glucose, but 10−4m sodium arsenite had little effect when the substrate was glycerol.

Type
Research Article
Information
Parasitology , Volume 48 , Issue 1-2 , May 1958 , pp. 165 - 183
Copyright
Copyright © Cambridge University Press 1958

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References

Agosin, M. & Brand, T. von (1954). The influence of puromycin on the carbohydrate metabolism of Trypanosoma equiperdum. Antibiotics & Chemotherapy, 4, 624–32.Google ScholarPubMed
Brand, T. von & Tobie, E. J. (1948). Further observations on the influence of cyanide on some trypanosomes. J. cell. comp. Physiol. 31, 49–68.CrossRefGoogle Scholar
Brand, T. von, Tobie, E. J. & Mehlman, B. (1950). The influence of some sulfhydryl inhibitors and of fluoroacetate on the oxygen consumption of some trypanosomes. J. cell. comp. Physiol. 35, 273–300.Google Scholar
Brand, T. von, Tobie, E. J., Mehlman, B. & Weinbach, E. C. (1953). Observations on the metabolism of normal and arsenic-resistant Trypanosoma gambiense. J. cell. comp. Physiol. 41, 1–22.CrossRefGoogle Scholar
Brand, T. von, Weinbach, E. C. & Tobie, E. J. (1955). Comparative studies on the metabolism of the culture form and bloodstream form of Trypanosoma gambiense. J. cell. comp. Physiol. 45, 421–33.CrossRefGoogle Scholar
Harvey, S. C. (1949). The carbohydrate metabolism of Trypanosoma hippicum. J. biol. Chem. 179, 435–53.Google Scholar
Marshall, P. B. (1948). The glucose metabolism of Trypanosoma evansi and the action of trypanocides. Brit. J. Pharmacol. 3, 8–14.Google ScholarPubMed
Michaelis, L. & Salomon, K. (1930). Respiration of mammalian erythrocytes. J. gen. Physiol. 13, 683–93.Google Scholar
Moulder, J. W. (1948 a). The oxidative metabolism of Trypanosoma lewisi in a phosphate–saline medium. J. infect. Dis. 83, 33–41.Google Scholar
Moulder, J. W. (1948 b). Changes in the glucose metabolism of Trypanosoma lewisi during the course of infection in the rat. J. infect. Dis. 83, 42–9.CrossRefGoogle ScholarPubMed
Reiner, L., Smythe, C. V. & Pedlow, J. T. (1936). On the glucose metabolism of trypanosomes (Trypanosoma equiperdum and Trypanosoma lewisi). J. biol. Chem. 113, 75–88.CrossRefGoogle Scholar
Ryley, J. F. (1951). Studies on the metabolism of the Protozoa. 1. Metabolism of the parasitic flagellate, Trypanosoma lewisi. Biochem. J. 49, 577–85.Google Scholar
Ryley, J. F. (1956). Studies on the metabolism of the Protozoa. 7. Comparative carbohydrate metabolism of eleven species of trypanosome. Biochem. J. 62, 215–22.CrossRefGoogle Scholar
Thurston, J. P. (1957). The effect of oxygen uptake of Trypanosoma lewisi and Trypanosoma equiperdum, with special reference to oxygen consumption in the presence of amino-acids. Parasitology, 48, 149–64.CrossRefGoogle Scholar