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The effect of drugs on the cell cycle of Giardia intestinalis

Published online by Cambridge University Press:  06 April 2009

G. F. Hoyne ,
P. F.L. Boreham ,
P. G. Parsons ,
C. Ward  and
B. Biggs
G. F. Hoyne
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland, Australia 4006
P. F.L. Boreham
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland, Australia 4006
P. G. Parsons
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland, Australia 4006
C. Ward
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland, Australia 4006
B. Biggs
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland, Australia 4006
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Summary

Flow cytometric analysis of the binucleated protozoan parasite Giardia intestinalis gave DNA histograms with a broad Gl peak and a definable G2 + M peak with twice the DNA content of Gl. Twenty-four hour treatment with metronidazole arrested cell cycle progression of susceptible trophozoites in the G2 + M phase, but had no effect, even at toxic doses, on the DNA histogram of a line selected for resistance to metronidazole. Furazolidone was inhibitory to both stocks, causing an arrest in the S and G2 + M phases. Inhibitors of the mammalian cell cycle were also tested. Hydroxyurea, which blocks mammalian cells in Gl/S, and razoxane, which blocks in G2 + M, arrested trophozoites in the G2 + M phase whereas colchicine and gamma-irradiation had little or no effect on the cell cycle of G. intestinalis. These results suggest that the cell cycle of G. intestinalis may be controlled in a different manner from mammalian cells.

Keywords

Giardia intestinaliscell cycleflow cytometrymetronidazolefurazolidone.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 333 - 339
Copyright
Copyright © Cambridge University Press 1989

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