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Early pulmonary response in rats infected with Trichinella spiralis

Published online by Cambridge University Press:  16 October 2006

S. M. VENTURIELLO ,
M. L. VERZOLETTI ,
S. N. COSTANTINO ,
M. A. FORASTIERO  and
M. E. ROUX
S. M. VENTURIELLO
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Humoral Immunity Studies Institute CONICET, Junín 956, (1113) Buenos Aires, Argentina
M. L. VERZOLETTI
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Humoral Immunity Studies Institute CONICET, Junín 956, (1113) Buenos Aires, Argentina
S. N. COSTANTINO
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Humoral Immunity Studies Institute CONICET, Junín 956, (1113) Buenos Aires, Argentina
M. A. FORASTIERO
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Humoral Immunity Studies Institute CONICET, Junín 956, (1113) Buenos Aires, Argentina
M. E. ROUX
Affiliation:
Chair of Physiopathology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Humoral Immunity Studies Institute CONICET, Junín 956, (1113) Buenos Aires, Argentina
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Abstract

The migratory stage of Trichinella spiralis, the newborn larva, travels along the pulmonary microvascular system on its way to the striated muscle cells. In the present study, an important inflammatory reaction was observed on days 5 and 14 post-infection (p.i.) in the lungs of infected rats. This inflammation was characterized by a Th2 cell phenotype of hyperplastic bronchus-associated lymphoid tissue and by goblet cell hyperplasia. Among the inflammatory cells were eosinophils and mast cells scattered over the pulmonary parenchyma. On day 5 p.i. the number of IgE+, CD4+ and CD5+ cells in the bronchus-associated lymphoid tissue were increased and IgE-secreting lung cells were also detected. At the end of the migratory phase of the infection (day 14 p.i.), only IgE+ cells were detected in high numbers and in the bronchoalveolar lavage fluid, an increment in the total IgE levels as well as the presence of IgE and IgA anti-larvae surface were also detected. In cytotoxicity assays, cells from the bronchoalveolar lavage had considerable biological activity since they were able to kill the larvae even in the absence of specific antibodies. These results show that the lung is an organ involved in the immune response developed early during a T. spiralis infection and suggest its importance in the protection of the host.

Keywords

trichinellosismigratory larvaelung inflammationbronchus-associated lymphoid tissue
Type
Research Article
Information
Parasitology , Volume 134 , Issue 2 , February 2007 , pp. 281 - 288
Copyright
2006 Cambridge University Press

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