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Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum

Published online by Cambridge University Press:  17 June 2011

Y. N. DUAN ,
H. Y. QIAN ,
Y. W. QIN ,
D. D. ZHU ,
X. X. HE ,
Q. ZHOU ,
Y. N. YANG ,
J. BAO ,
J. R. FENG  and
W. SUN
...Show all authors
Y. N. DUAN*
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
H. Y. QIAN
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
Y. W. QIN
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
D. D. ZHU
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
X. X. HE
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
Q. ZHOU
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
Y. N. YANG
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
J. BAO
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
J. R. FENG
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
W. SUN
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
J. L. CHEN
Affiliation:
Department of Parasitology and Microbiology, School of Medicine, Nantong University; Nantong, Jiangsu Province, 226001, People's Republic of China
*
*Corresponding author: Tel: +86 513 85051738. E-mail: [email protected]
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Summary

In order to investigate the dynamics of Septin4 (Sept4) expression and its function in the formation of fibrotic livers in mice infected with Schistosoma japonicum, we constructed the mouse model of S. japonicum egg-induced liver fibrosis for 24 weeks. Immunohistochemical staining, qRT-PCR and Western blot were used to detect the expression of Sept4 and α-smooth muscle actin (α-SMA). We found Sept4 localized in the perisinusoidal space where hepatic stellate cells (HSCs) distribute in the periphery of circumoval granulomas and the portal venule. The expression of Sept4 and α-SMA had a similar significant tendency of an up-regulation to a peak at 12 weeks post-infection (p.i.) followed by a down-regulation. At 24 weeks p.i. both were at a low level. These results suggest that Sept4 and α-SMA may interact together in HSCs. Based on this evidence, we hypothesize that Sept4 seems to be involved in the formation of inflammatory granulomata and subsequent liver fibrosis by regulating HSCs activation.

Keywords

Septin4hepatic stellate cellsSchistosoma japonicumliver fibrosis
Type
Research Article
Information
Parasitology , Volume 138 , Issue 8 , July 2011 , pp. 1003 - 1010
Copyright
Copyright © Cambridge University Press 2011

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