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DNA probes for the detection of Babesia caballi

Published online by Cambridge University Press:  06 April 2009

E. S. Posnett  and
R. E. Ambrosio
E. S. Posnett
Affiliation:
Molecular Biology Section, Veterinary Research Institute, Onderstepoort 0110, South Africa
R. E. Ambrosio
Affiliation:
Molecular Biology Section, Veterinary Research Institute, Onderstepoort 0110, South Africa
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Extract

A genomic library of Babesia caballi DNA was constructed in the plasmid vector pUC13. The specificity of the clones for B. caballi was established by the lack of hybridization to Babesia equi, Babesia bovis, Babesia bigemina and equine DNA. Two probes, pBC11 and pBC191, were isolated that could detect 0·25 ng and 0·125 ng of B. caballi DNA, corresponding to a parasitaemia of 0·12% and 0·06% respectively. pBC191 could detect B. caballi parasites in the blood of an experimentally infected horse as well as in naturally infected horses.

Keywords

equine babesiosisBabesia caballiDNA probetandem repeat
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 357 - 365
Copyright
Copyright © Cambridge University Press 1991

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