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Differentiation of isolates in the genus Steinernema (Nematoda: Steinernematidae).by random amplified polymorphic DNA fragments and morphological characters

Published online by Cambridge University Press:  06 April 2009

J. Liu  and
R. E. Berry
J. Liu
Affiliation:
Department of Entomology, Oregon State University, Corvallis, OR 97331–2907, USA
R. E. Berry
Affiliation:
Department of Entomology, Oregon State University, Corvallis, OR 97331–2907, USA
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Summary

We combined polymerase chain reaction (PCR) amplification of DNA sequences and important morphological characters as a technique to differentiate nematode isolates in the genus Steinernema. Five decamer oligonucleotide primers were used to generate random amplified polymorphic DNA (RAPD) fragments from 11 nematode isolates. The primers generated 8–12 fragments, ranging from 220 to 1300 bp in size. Reproducible amplified DNA fragments of 11 isolates showed obviously inter- or intra-specific polymorphisms, enabling us to differentiate easily the nematode species and isolates. Combining RAPD–PCR fragments with the examination of morphological characters of infective juveniles and 1st-generation males, we identified isolate OH1S, collected from Newport, Oregon, as S.feltiae; isolate OS21, collected from Grants Pass, Oregon, belonged to a previously undescribed species. Our study may provide a rapid and reliable method for the identification of Steinernema nematodes.

Keywords

entomopathogenic nematodesSteinernema spp.identificationRAPD–PCRmorphological characters.
Type
Research Article
Information
Parasitology , Volume 111 , Issue 1 , July 1995 , pp. 119 - 125
Copyright
Copyright © Cambridge University Press 1995

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