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Differential virulence of camel Trypanosoma evansi isolates in mice

Published online by Cambridge University Press:  24 January 2018

Christine M. Kamidi ,
Joanna Auma ,
Paul O. Mireji ,
Kariuki Ndungu ,
Rosemary Bateta ,
Richard Kurgat ,
Collins Ouma ,
Serap Aksoy  and
Grace Murilla
Christine M. Kamidi*
Affiliation:
Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Maseno, Kenya Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA
Joanna Auma
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya
Paul O. Mireji
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya Centre for Geographic Medicine Research Coast, Kenya Medical Research Institute, P.O. Box 428, Kilifi, Kenya
Kariuki Ndungu
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya
Rosemary Bateta
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya
Richard Kurgat
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya
Collins Ouma
Affiliation:
Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Maseno, Kenya
Serap Aksoy
Affiliation:
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA
Grace Murilla*
Affiliation:
Biotechnology Research Institute (BioRI) – Kenya Agricultural and Livestock Research Organization (KALRO), P.O. Box 362, Kikuyu 00902, Kenya Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA
*
Author for correspondence: Christine M. Kamidi and Grace Murilla, E-mail: [email protected], [email protected]
Author for correspondence: Christine M. Kamidi and Grace Murilla, E-mail: [email protected], [email protected]
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Abstract

This study assessed the virulence of Trypanosoma evansi, the causative agent of camel trypanosomiasis (surra), affecting mainly camels among other hosts in Africa, Asia and South America, with high mortality and morbidity. Using Swiss white mice, we assessed virulence of 17 T. evansi isolates collected from surra endemic countries. We determined parasitaemia, live body weight, packed cell volume (PCV) and survivorship in mice, for a period of 60 days’ post infection. Based on survivorship, the 17 isolates were classified into three virulence categories; low (31–60 days), moderate (11–30 days) and high (0–10 days). Differences in survivorship, PCV and bodyweights between categories were significant and correlated (P < 0.05). Of the 10 Kenyan isolates, four were of low, five moderate and one (Type B) of high virulence. These findings suggest differential virulence between T. evansi isolates. In conclusion, these results show that the virulence of T. evansi may be region specific, the phenotype of the circulating parasite should be considered in the management of surra. There is also need to collect more isolates from other surra endemic regions to confirm this observation.

Keywords

Packed cell volumesurvivalTrypanosoma evansivirulence.
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1235 - 1242
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Copyright
Copyright © Cambridge University Press 2018 

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