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Dietary lipids containing gangliosides reduce Giardia muris infection in vivo and survival of Giardia lamblia trophozoites in vitro

Published online by Cambridge University Press:  13 May 2004

M. SUH ,
M. BELOSEVIC  and
M. T. CLANDININ
M. SUH
Affiliation:
Nutrition and Metabolism Research Group, Departments of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
M. BELOSEVIC
Affiliation:
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2E9, Canada.
M. T. CLANDININ
Affiliation:
Nutrition and Metabolism Research Group, Departments of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2E9, Canada Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
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Abstract

We examined whether a ganglioside supplemented diet affected the course of Giardia muris infection in mice and survival of Giardia lamblia trophozoites in vitro. Female CD-1 mice were fed 1 of 5 experimental diets: standard lab chow as a control diet; semi-synthetic diets containing 20% (w/w) triglyceride based on the fat composition of a conventional infant formula; triglyceride diet; triglyceride diet containing a low level of ganglioside (0·1% w/w); and triglyceride diet containing a high level of ganglioside (1·0% w/w of diet). After 2 weeks of feeding, mice were inoculated with G. muris by gastric intubation and fed the experimental diets during the course of the infection. Cysts released in the faeces and trophozoites present in the small intestine were enumerated at various times post-infection. The average cyst output and the number of trophozoites during the course of the infection in mice fed ganglioside-containing diet were found to be significantly lower (3-log10 reduction) compared to animals fed control diets. The results of in vitro growth studies indicated that gangliosides may be directly toxic to the parasites. Thus, gangliosides have a protective effect against G. muris infection in vivo and affect the survival of G. lamblia trophozoites in vitro.

Keywords

dietgangliosideGiardiaprotozoaintestineinfection
Type
Research Article
Information
Parasitology , Volume 128 , Issue 6 , June 2004 , pp. 595 - 602
Copyright
2004 Cambridge University Press

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