Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T21:42:57.121Z Has data issue: false hasContentIssue false
  • English
  • Français

Developmental cycles of Trypanosoma (Schizotrypanum) cruzi (Chagas, 1909) in mouse peritoneal macrophages in vitro

Published online by Cambridge University Press:  06 April 2009

Kazem Behbehani
Kazem Behbehani
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT
Get access Rights & Permissions [Opens in a new window]

Extract

Mouse peritoneal macrophage cells suspended in a TC 199 calf serum medium and cultured in Leighton tubes, were infected with Trypanosoma (Schizotrypanum) cruzi culture forms in order to study the development of this parasite in vitro. Three cycles of development were thought to occur: 1. Epimastigotes or trypomastigotes were taken up by macrophages and transformed into amastigotes. These multiplied by binary fission and ruptured the infected cell after 4‐5 days. Released amastigotes were taken up by uninfected macrophages and the cycle was repeated. 2. A small proportion of intracellular amastigotes developed into ‘ovoid forms’ which transformed progressively into promastigotes, epimastigotes and trypomastigotes. 3. Some amastigotes became ‘round forms’ from which sphaeromastigotes developed. These transformed directly into trypomastigotes without the formation of epimastigotes.

Type
Research Article
Information
Parasitology , Volume 66 , Issue 2 , April 1973 , pp. 343 - 353
Copyright
Copyright © Cambridge University Press 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brack, C., (1968). Elektronenmikroskopische Untersuchungen zum lebenszyklus von Trypanosoma cruzi. Acta Tropica 25, 289–356.Google ScholarPubMed
Brumpt, E., (1912). Le Trypanosoma cruzi evolve chez Conorhinus megistus, Cimex lectularius, Cimex boueti et Ornithodorus moubata. Cycle evolutif de oe parasite. Bulletin de la Société de Pathologie exotique 5, 360–7.Google Scholar
Camargo, E. P., (1964). Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyolic trypanosomes in liquid media. Revista do Institute de Medicina Tropical de Sao Paulo 6, 93–100.Google ScholarPubMed
Hartmann, M., (1910). Notiz über eine weitere Art der schizogonie bei Schizotrypanum cruzi (Chagas). Archives de Parasitologie 20, 361–3.Google Scholar
Hartmann, M., (1917). über die Schizogonie von Schizotrypanum cruzi. Archives de Parasitologie 38, 113–16.Google Scholar
Hoare, C. A., & Wallace, F. G., (1966). Developmental stages of trypanosomatid flagellates: a new terminology. Nature, London 212, 1385–6.CrossRefGoogle Scholar
Kofoid, C. A., Wood, F. D., & McNeil, E., (1935). The cycle of Trypanosoma cruzi in tissue culture of embryonic heart muscle. University of California Publications in Zoology 41, 23–4.Google Scholar
Lamy, L. H., (1970). Transformation intracellulaire des forms amastigotes de Leishmania donovani et Trypanosoma cruzi en formes mastigotes. Protistologica 6, 457–66.Google Scholar
Muniz, J., & De Freitas, G., (1946). Realizacao ‘in vitro’ do ciclo do ‘S. cruzi’ no vertebrado, em meios de caldo-liquido peritoneal. Revista Brasileira de Biologia 6, 467–84.Google Scholar
Nakamura, M., (1967). Cultivation of Trypanosoma cruzi in a protein-free dialysate medium. Proceedings of the Society for Experimental Biology and Medicine 125, 779–80.CrossRefGoogle Scholar
Petana, W. P., (1971). Taxonomic classification of developmental stages of Trypanosoma (Schizotrypanum) cruzi. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 270–1.CrossRefGoogle ScholarPubMed
Rodriguez, E., & Marinkelle, C. J., (1970). Trypanosoma cruzi: development in tissue culture. Experimental Parasitology 27, 78–87.CrossRefGoogle ScholarPubMed
Romana, C., & Meyer, H., (1942). Estudo do ciclo evolutivo do ‘Schizotrypanum cruzi’ em cultura de tecidos de embriao de golinha. Memorias do Institute Oswaldo Cruz. 27, 19–27.CrossRefGoogle Scholar
Seah, S., & Marsden, P. D., (1970). Complement-fixation test in Trypanosoma rhodesiense infection with culture Trypanosoma cruzi as antigen. Transactions of the Royal Society of Tropical Medicine and Hygiene 64, 279–83.CrossRefGoogle ScholarPubMed
Silva, L. H. P. Da (1959). Observacoes sobre o ciclo evolutivo do Trypanosoma cruzi. Revista do Instituto de Medicina Tropical de Sao Paulo 1, 99–118.Google Scholar
Tang, C. C., (1953). Cultivation of Trypanosoma cruzi in tissue culture. Chinese Medical Journal 71, 115–26.Google Scholar
Wenyon, C. M., (1926). Protozoology: A Manual for Medical Men, Veterinarians and Zoologists, 1st ed., London: Baillière, Tindall and Cox. p. 492.CrossRefGoogle Scholar
Wood, S. F., (1951). Development of Arizona Trypanosoma cruzi in mouse muscle. American Journal of Tropical Medicine and Hygiene 31, 1–11.CrossRefGoogle ScholarPubMed
Zuckerman, A., (1953). Culture of mononuclear cells from rat peritoneal exudate. Proceedings of the Society for Experimental Biology and Medicine 82, 469–71.CrossRefGoogle ScholarPubMed