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Development of Sarcocystis falcatula in cell cultures demonstrates that it is different from Sarcocystis neurona

Published online by Cambridge University Press:  01 March 1999

D. S. LINDSAY ,
J. P. DUBEY ,
K. M. HORTON  and
D. D. BOWMAN
D. S. LINDSAY
Affiliation:
Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, 1410 Prices Fork Road, Blacksburg, Virginia 24061-0342, USA
J. P. DUBEY
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705-2350, USA
K. M. HORTON
Affiliation:
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA
D. D. BOWMAN
Affiliation:
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA
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Abstract

The development of Sarcocystis falcatula merozoites in bovine turbinate (BT) cell cultures is described and compared with development of Sarcocystis neurona merozoites. Merozoites of S. falcatula entered BT cell cultures and increased in size until 3 days post-inoculation when the nucleus of some merozoites developed lobes. Developing schizonts present at 4 days contained a lobed nucleus or appeared multinucleate. A single mature schizont was observed 4 days p.i. Schizonts were numerous 5 and 6 days p.i. Merozoites were produced from blastophores on the schizont. S. neurona merozoites developed to mature schizonts by 3 days p.i. in BT cells and a residual body was often present. Transmission electron microscopy revealed that S. falcatula merozoites possessed more micronemes than did S. neurona merozoites. Our study demonstrates that S. falcatula and S. neurona are not the same parasite.

Keywords

Sarcocystis falcatulaSarcocystis neuronacoccidiaschizontsmerozoitescell culture
Type
Research Article
Information
Parasitology , Volume 118 , Issue 3 , March 1999 , pp. 227 - 233
Copyright
1999 Cambridge University Press

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