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Development of an immunochromatographic device to detect antibodies for rapid diagnosis of human angiostrongyliasis

Published online by Cambridge University Press:  06 November 2019

Chalermchai Somboonpatarakun ,
Pewpan M. Intapan ,
Lakkhana Sadaow ,
Rutchanee Rodpai ,
Oranuch Sanpool  and
Wanchai Maleewong Open the ORCID record for Wanchai Maleewong [Opens in a new window]
Chalermchai Somboonpatarakun
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
Pewpan M. Intapan
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
Lakkhana Sadaow
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
Rutchanee Rodpai
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
Oranuch Sanpool
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
Wanchai Maleewong*
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen40002, Thailand
*
Author for correspondence: Wanchai Maleewong, E-mail: [email protected]
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Abstract

Cerebral angiostrongyliasis is a central nervous system disease caused by Angiostrongylus cantonensis and can produce eosinophilic meningitis or meningoencephalitis in humans. Sero-immunological techniques, such as the enzyme-linked immunosorbent assay (ELISA) and immunoblotting, are most commonly used for the diagnosis of human angiostrongyliasis. However, diagnosis in remote areas remains problematic because sophisticated equipment and specialized skills are required. To overcome, we have developed the immunochromatographic test (ICT) kit for rapid serological diagnosis of angiostrongyliasis through the detection of anti-A. cantonensis-specific antibodies in human serum. A recombinant A. cantonensis galectin-2 (rAcGal2) from young adult female worms was used as an antigen for the ICT kit development. Diagnostic values were evaluated and compared using the ELISA. The sensitivity, specificity and positive and negative predictive values of the ICT kit were 87.0, 96.5, 94.6 and 91.4%, respectively, and those of the ELISA were 91.0, 97.2, 95.8 and 94.0%, respectively. The concordance of the ICT kit was 93.9%. We, thus, determined that the ICT kit is sensitive and specific and provides reliable diagnostic results. It is rapid and simple to perform and can be utilized for both point-of-care diagnosis in the bedside laboratory and epidemiological surveys in endemic regions where access to diagnostic equipment is limited.

Keywords

AngiostrongyliasisAngiostrongylus cantonensisimmunochromatographic test kitrecombinant proteinserodiagnosis
Type
Research Article
Information
Parasitology , Volume 147 , Issue 2 , February 2020 , pp. 194 - 198
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Copyright
Copyright © Cambridge University Press 2019

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References

Barratt, J, Chan, D, Sandaradura, I, Malik, R, Spielman, D, Lee, R, Marriott, D, Harkness, J, Ellis, J and Stark, D (2016) Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen. Parasitology 143, 1087–1118.CrossRefGoogle ScholarPubMed
Cross, JH (1978) Clinical manifestations and laboratory diagnosis of eosinophilic meningitis syndrome associated with angiostrongyliasis. The Southeast Asian Journal of Tropical Medicine and Public Health 9, 161–170.Google ScholarPubMed
Cross, JH and Chen, ER (2007) Angiostrongyliasis. In Murrell, KD and Fried, B (eds), Food-Borne Parasitic Zoonoses: Fish and Plant Borne Parasites. New York, USA: Springer, pp. 263–290.CrossRefGoogle Scholar
Eamsobhana, P and Yong, HS (2009) Immunological diagnosis of human angiostrongyliasis due to Angiostrongylus cantonensis (Nematoda: Angiostrongylidae). International Journal of Infectious Diseases 13, 425–431.CrossRefGoogle Scholar
Eamsobhana, P, Lim, PE and Yong, HS (2013) Genetic diversity of the rat lungworm, Angiostrongylus cantonensis, the major cause of eosinophilic meningitis. Hawai‘i Journal of Medicine & Public Health 72, 15–17.Google ScholarPubMed
Eamsobhana, P, Tungtrongchitr, A, Wanachiwanawin, D and Yong, HS (2018) Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis. International Journal of Infectious Diseases 73, 69–71.CrossRefGoogle ScholarPubMed
Elkins, DB, Haswell-Elkins, M and Anderson, RM (1986) The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 774–792.CrossRefGoogle Scholar
Galen, RS (1980) Predictive value and efficiency of laboratory testing. Pediatric Clinics of North America 27, 861–869.CrossRefGoogle ScholarPubMed
Graeff-Teixeira, C, da Silva, AC and Yoshimura, K (2009) Update on eosinophilic meningoencephalitis and its clinical relevance. Clinical Microbiology Reviews 22, 322–348.CrossRefGoogle ScholarPubMed
Intapan, PM, Maleewong, W, Sawanyawisuth, K and Chotmongkol, V (2003) Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis. Parasitology Research 89, 425–429.CrossRefGoogle ScholarPubMed
Intapan, PM, Khotsri, P, Kanpittaya, J, Chotmongkol, V, Maleewong, W and Morakote, N (2008) Evaluation of IgG4 and total IgG antibodies against cysticerci and peptide antigens for the diagnosis of human neurocysticercosis by ELISA. Asian Pacific Journal of Allergy and Immunology 26, 237–244.Google Scholar
Jaroonvesama, N (1988) Differential diagnosis of eosinophilic meningitis. Parasitology Today 4, 262–266.CrossRefGoogle ScholarPubMed
Kirsch, S, Dekumyoy, P, Loscher, T and Haberl, RL (2008) A case of eosinophilic meningitis in Germany. Journal of Neurology 255, 1102–1103.CrossRefGoogle ScholarPubMed
Maleewong, W, Sombatsawat, P, Intapan, PM, Wongkham, C and Chotmongkol, V (2001) Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis. Asian Pacific Journal of Allergy and Immunology 19, 267–273.Google ScholarPubMed
Nuamtanong, S (1996) The evaluation of the 29 and 31 kDa antigens in female Angiostrongylus cantonensis for serodiagnosis of human angiostrongyliasis. The Southeast Asian Journal of Tropical Medicine and Public Health 27, 291–296.Google ScholarPubMed
Pien, FD and Pien, BC (1999) Angiostrongylus cantonensis eosinophilic meningitis. International Journal of Infectious Diseases 3, 161–163.CrossRefGoogle ScholarPubMed
Podwall, D, Gupta, R, Furuya, EY, Sevigny, J and Resor, SR (2004) Angiostrongylus cantonensis meningitis presenting with facial nerve palsy. Journal of Neurology 251, 1280–1281.CrossRefGoogle ScholarPubMed
Punyagupta, S (1979) Angiostrongyliasis: clinical features and human pathology. In Cross, JH (ed.), Studies on Angiostrongyliasis in Eastern Asia and Australia. Taipei, Taiwan: U.S. Naval Medical Research Unit No. 2, pp. 138–150.Google Scholar
Slom, TJ, Cortese, MM, Gerber, SI, Jones, RC, Holtz, TH, Lopez, AS, Zambrano, CH, Sufit, RL, Sakolvaree, Y, Chaicumpa, W, Herwaldt, BL and Johnson, S (2002) An outbreak of eosinophilic meningitis caused by Angiostrongylus cantonensis in travelers returning from the Caribbean. The New England Journal of Medicine 346, 668–675.CrossRefGoogle ScholarPubMed
Somboonpatarakun, C, Intapan, PM, Sanpool, O, Wongkham, C and Maleewong, W (2019) Application of recombinant Angiostrongylus cantonensis galectin-2 protein for serodiagnosis of human angiostrongyliasis by immunoblotting. The American Journal of Tropical Medicine and Hygiene 101, 851–858.CrossRefGoogle ScholarPubMed
Tesana, S, Srisawangwong, T, Sithithaworn, P, Laha, T and Andrews, R (2009) Prevalence and intensity of infection with third stage larvae of Angiostrongylus cantonensis in mollusks from Northeast Thailand. The American Journal of Tropical Medicine and Hygiene 80, 983–987.CrossRefGoogle ScholarPubMed
Thiengo, SC, Simões Rde, R, Fernandez, MA and Maldonado, A Jr (2013) Angiostrongylus cantonensis and rat lungworm disease in Brazil. Hawai‘i Journal of Medicine & Public Health 72, 18–22.Google Scholar
Tsai, HC, Liu, YC, Kunin, CM, Lee, SS, Chen, YS, Lin, HH, Tsai, TH, Lin, WR, Huang, CK, Yen, MY and Yen, CM (2001) Eosinophilic meningitis caused by Angiostrongylus cantonensis: report of 17 cases. The American Journal of Medicine 111, 109–114.CrossRefGoogle ScholarPubMed
Tsai, HC, Lee, SS, Huang, CK, Yen, CM, Chen, ER and Liu, YC (2004) Outbreak of eosinophilic meningitis associated with drinking raw vegetable juice in southern Taiwan. The American Journal of Tropical Medicine and Hygiene 71, 222–226.CrossRefGoogle ScholarPubMed
Tsai, HC, Chen, YS and Yen, CM (2013) Human parasitic meningitis caused by Angiostrongylus cantonensis infection in Taiwan. Hawai‘i Journal of Medicine & Public Health 72, 26–27.Google ScholarPubMed
Wang, QP, Chen, XG and Lun, ZR (2007) Invasive freshwater snail, China. Emerging Infectious Diseases Journal 13, 1119–1120.CrossRefGoogle Scholar
Wang, QP, Lai, DH, Zhu, XQ, Chen, XG and Lun, ZR (2008) Human angiostrongyliasis. The Lancet Infectious Diseases 8, 621–630.CrossRefGoogle ScholarPubMed
Zhou, R and Caspi, RR (2010) Ocular immune privilege. F1000 Biology Reports 2, 3.Google ScholarPubMed